به شکل جدول

۱

(Ketogenic or Ketosteroids)

۱ months at -20°C

• Birth control pills that contain estrogen
• Certain antibiotics
• Glucocorticoids

۲

۶ months at -20°C

Keep in mind that anyone who has been diagnosed with CAH should be tested periodically so the condition can be monitored. Changes in 17-OH progesterone levels may indicate a need for an adjustment in treatment.

(۲-mercaptoethanol)

Longer time at -20°C

(۵-Hydroxyindoleacetic Acid)

۲ weeks at -20°C

(dark place)

(Angiotensin Converting Enzyme)

۶ months at -20°C

Be separated quickly.

Note:  Measurement of ACE activity for the evaluation of sarcoidosis is not reliable when ACE inhibitors are present. Serum ACE activity is markedly reduced in patients on ACE inhibitor therapy.

۲ months at -20°C

(AChR Ab)

۱ year at -20°C

Be separated quickly. Repeated freeze-thaw cycles should be avoided.

۸

( Prostatic)

۲۴ hrs. at 2-8°C

۶ months at -20°C

Be separated quickly. Repeated freeze-thaw cycles should be avoided.

•Abstain using of cefotaxim few days prior to collection.

•Notice using of Alglucerase, Androgens, Clofibrate, Fluorides, goserelin, Heparins, Ketoconazole, Oxalates … that affected ACE level.

•Avoid  prostate massage & rectal exam 2 days prior to collection.

۹

CRITICAL FROZEN

Be separated quickly. Repeated freeze-thaw cycles should be avoided.

•Abstain using of cefotaxim few days prior to collection.

•Notice using of Alcohol, Anabolic steroids, Androgens, Clofibrate,  Fluorides, Oxalates, Phosphates  … that affected ACE level.

•Avoid  prostate massage & rectal exam 2 days prior to collection.

(Adrenocorticotropic Hormone)

CRITICAL FROZEN

Be separated quickly and transport on ice. Refrigerated centrifuge must be used.

Serum, tissue or urine. Heparinized plasma.

(Adenosine Deaminase)

۱ months at -20°C

An ADA test may be ordered when a person has an accumulation of pleural fluid and has signs or symptoms that suggest  TB (tuberculous meningitis), such as:

•Chronic cough, sometimes with bloody sputum

•Fever, chills

•Night sweats

•Unexplained weight loss

•Chest pain

U(R&24H)

۳ months at -20°C

In body fluids: Aids in discriminating transudate from exudate in the evaluation of ascites.

۲ months at  -۲۰°C

Be separated quickly

۱ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Abstain using of anti blood pressure, diuretic, … ۲ weeks prior to collection.

EDTA plasma.

( Aldosterone/Renin Activity Ratio) or Aldosterone and Renin, Direct with Ratio  for diagnosing hyperaldosteronism.

۲ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Specimens collected in EDTA or sodium fluoride/potassium oxalate.

۳ months at -20°C

۲ months at  -۲۰°C

Repeated freeze-thaw cycles should be avoided.

Avoid using of cefotaxim prior to collection.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Others: 1

۱ months at -20°C

Plasma sample be separated quickly.

Infants and children: Draw specimen prior to feeding or 2-3 hours after a meal.

Urine must be fresh.

In CSF: Aid in the diagnosis of selected neurotransmitter disorders (eg, glycine encephalopathy, brain serine deficiency).

In Urine: Screen for disorders of amino acids transport (eg, cystinuria, lysinuric protein intolerance, HHH syndrome).  Also useful to evaluate renal tubular function.

Clinical information is needed for appropriate interpretation. Additional required information includes age, gender, diet (e.g., TPN therapy), drug therapy, and family history.

۳ months at -20°C

Refrigerated Centrifuge

Serum or blood. Non-frozen specimens. Specimens containing oxalate or citrate

•Investigating and monitoring treatment for in born errors of metabolism. (pecifically used to assist in diagnosing infant Reye’s syndrome)

•Evaluating patients with advanced liver disease

۳ months at -20°C

U(R&24H)

۷ days at 2-8°C

S: 1 month at -20°C

U: 7 days at -20°C

Repeated freeze-thaw cycles should be avoided.

(Antinuclear Antibody)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

(Anti-Neutrophil Cytoplasmic Antibody)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

• A reflex panel (ANCA-Associated Vasculitis Profile (ANCA/MPO/PR-3) )is available for the workup of suspected vasculitis.

• Recommended in the differential evaluation of autoimmune diseases with renal involvement (SLE and anti-glomerular basement membrane disease.

• Recommended adjunct diagnostic tool to differentiate ulcerative colitis from Crohn disease in IBD.

۳ months at -20°C

Sample collection 1 week before or after menstruation.

Specimen should be collected between 6-10 a.m.

۳ months at -20°C

First-line panel for the evaluation of autoimmune liver disease (ALD) is ASMA, Anti Mitochondrial Ab, Anti LKM1 and ASMA titer.

For longer time at -20°C

•Investigation of suspected roundworm infections

For longer time at -20°C

•Investigation of suspected roundworm infections

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

( IgA )

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

• Preferred screening test for individuals with suspected celiac disease is Celiac Disease Reflexive Cascade  or Tissue Transglutaminase (tTG) Antibody, IgA . (IgA testing recommended to identify IgA competence)

• May aid in monitoring adherence to gluten-free diet in celiac disease confirmed patients.

 ( IgG )

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

• Preferred screening test is Celiac Disease Reflexive Cascade  or Tissue Transglutaminase (tTG) Antibody, IgG .  (IgA testing recommended to identify IgA deficiency (

• May aid in monitoring adherence to gluten-free diet in celiac disease confirmed patients.

( Glomerular Basement Membrane Antibody)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۲ months at -20°C

• If pursuing antibody testing to dermine autoimmune diabetes mellitus, perform at least 2 antibody tests. In most cases, use glutamic acid decarboxylase antibody in combination with another antibody test. Other antibody tests include Glutamic Acid Decarboxylase Antibody , IA-2 Antibody , Islet Cell Cytoplasmic Antibody, IgG , and Zinc Transporter 8 Antibody.

• Do not use to differentiate type 1 DM from type 2 DM, for most cases.

(Liver Kidney Microsomal Type 1 Antibodies)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

(AMH)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

AMH may be ordered when a woman has signs and symptoms of polycystic ovarian syndrome (PCOS)

۳ months at -20°C

(  Ribonucleoprotein Autoantibodies)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

•Useful in the differential diagnosis of connective tissue diseases with or without myopathy.

•RNP antibody is seen in 95-100 percent of mixed connective tissue disease and is considered specific for this syndrome if other antibodies are negative; RNP is also present in 20-30 percent of systemic lupus erythematosus and 15-25 percent of progressive systemic sclerosis. RNP antigens also contain epitopes that are immunologically identical to free Smith antigens, therefore, the Smith antibody response must be considered when interpreting RNP results

(Anti-Scleroderma Antibodies / Topoisomerase 1)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

•As a follow-up test when sperm agglutination is noted in the ejaculate

•In men with a history of testicular trauma, biopsy, vasectomy reversal, genital tract infection, or

obstructive lesions of the male ductal system

(Anti-Sjögren’s-syndrome-related antigen A)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۱ months at -20°C

۱ months at -20°C

۱ months at -20°C

۱ months at -20°C

۴ hrs. at 2-8°C

۱۴ days at -20°C

(Anti-Saccharomyces cerevisiae antibodies)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Centrifuge 1000 rpm 10 min.

( Streptolysin O Antibody)

۱ months at -20°C

۱ months at -20°C

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۲ months at -20°C

Repeated freeze-thaw cycles should be avoided.

In urine: Preferred test to follow-up presumptive results. For general screening, Barbiturates Urine Screen with Reflex to Quantitation is preferred.

۱ months at -20°C

۳ months at -20°C

(U:2 months & CSF 2 weeks at -20°C)

Repeated freeze-thaw cycles should be avoided.

U: Frozen

RIA (titer)

۱ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

(dark place)

Repeated freeze-thaw cycles should be avoided.

(dark place)

(cell type & back type)

۱ week at 2-8°C

Do not freeze red cells.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۷ days at 2-8°C

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Clotted blood specimens.

۳ days at 2-8°C,

۱ months at -20°C

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۲ months at -20°C

Repeated freeze-thaw cycles should be avoided.

(C1-Esterase Inhibitor)

۲ weeks at -20°C

Repeated freeze-thaw cycles should be avoided.

(Complement Component 3)

۱ months at -20°C

Repeated freeze-thaw cycles should be avoided.

(Complement Component 4)

۱ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Body fluid tumor markers should NOT be used as sole evidence of malignancy for diagnostic purposes and should be reviewed in correlation with cytology, serum results, and other clinical evidence. Results should be interpreted with caution when considering literature-based sources of interpretive guidance, as alternative assays and/or instruments are frequently used between studies.

For longer time at -20°C

Repeated freeze-thaw cycles should be avoided.

For longer time at -20°C

For longer time at -20°C

For longer time at -20°C

Repeated freeze-thaw cycles should be avoided.

Do not use powdered gloves during collection.

(Fecal Calprotectin is an indicator of the presence of neutrophils in stool and is not specific for IBD. Other intestinal ailments including GI infections and colorectal cancer can result in elevated concentrations of calprotectin. The diagnosis of IBD cannot be established solely on the basis of a positive calprotectin result. Patients with IBD fluctuate between active and inactive stages of disease. Calprotectin results may also fluctuate. GI bleeding of as much as 100 mL per day will increase the fecal calprotectin concentration by only 15 µg/g.)

۳ months at -20°C

(Complete Blood count)

Flow Cytometry

۱ days at 2-8°C,

Incorrect anticoagulant

(Cyclic Citrullinated Peptide Antibody)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

For longer time at -20°C

(Total complement activity)

۱۴ days at -20°C

Repeated freeze-thaw cycles should be avoided.

Hemolysis

BF(CSF, Drain, Pancreatic, Pericardial, Peritoneal/Ascites or Pleural fluid)

۷ days at 2-8°C,

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۷ days at 2-8°C,

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۷ days at 2-8°C,

۳ months at -20°C

WB:

۳ days at 2-8°C

at 2-8°C, -20°C

WB:

at 2-8°C

Hemolysis, Lipemic

WB:

Hemolysis, Clotting, Frozen, Incorrect anticoagulant

pH= 2

Colorimetry

۱ months at -20°C

۱ months at -20°C

(dark place)

Repeated freeze-thaw cycles should be avoided.

۱ months at -20°C

(dark place)

Repeated freeze-thaw cycles should be avoided.

۳ days at 2-8°C

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

BAL, Serum, Amniotic fluid, CSF, Ocular fluid, Tissue, Urine

U 10 ml

For quatitative assay only

WB(E) is accepted

۸ hrs at RT,

۳ days at 2-8°C,

۱ yr at -20°C

Others:

۸ hrs at RT,

۳ days at 2-8°C,

۳ months at -20°C

Frozen

Others:

۲-۸°C

Calcium alginate tipped,

Wood and gel media swab.

BAL

۸ hrs at RT,

۳ days at 2-8°C,

۱ yr at -20°C

Others:

۸ hrs at RT,

۳ days at 2-8°C,

۳ months at -20°C

Formalinized fluids

۱ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۱ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۵ days at 2-8°C

۱ months at -20°C

۷ days at -20°C

۳ months at -20°C

specimens that are not separated from the red cells or clot within 6 hours.

۳ months at -20°C

Abstain stress, …

Sampling time between 6-8 am.

• Screen and diagnose primary and secondary adrenal insufficiency.

(Connecting Peptide of Insulin)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Specimens obtained through catheters used to infuse hyperalimentation fluid

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Random urine collections.

۳ months at -20°C

•DO NOT ORDER for cardiovascular disease (CVD) risk assessment. Test for CVD risk assessment is C-Reactive Protein, High Sensitivity.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Clot, maintain whole blood at 37°C untile clotting is complete. Separate serum from cells using a 37°C centrifuge, if possible.

Whole blood drawn in a pre-warmed (37°C) tube and syringe.

Frozen, storage at 2-8°C

(Oligoclonal bands)

۳ months at -20°C

۳ months at -20°C

۱ months at -20°C

Critical Frozen

Clinical information is needed for appropriate interpretation. Additional required information includes age, gender, diet (eg, TPN therapy), drug therapy, and family history.

Formalin 10%

Prepared slide,

۳ months at RT

Slide at RT

۱ months at -20°C

Centrifuge at 1500 rpm 15 min.

۲ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Collect specimen 8-12 hours (no earlier than 6 hours) after administration of oral dose.

۱ months at -20°C

For longer time at

-۲۰°C

Repeated freeze-thaw cycles should be avoided.

۷ days at at 2-8°C

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۲ months at -20°C

(amebiasis)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۱ months at -20°C

۲ months at -20°C

۱۲ hrs. at at 2-8°C

۲ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۲ months at -20°C

۱ months at -20°C

۱۴ days at -20°C,

۶ months at at at

-۸۰°C

Repeated freeze-thaw cycles should be avoided.

Be separated quickly/Two times centrifugation is preferred.

۱ week at 2-8°C

Plasma or serum; collection of specimen in sodium heparin tubes

۱ months at -20°C

Repeated freeze-thaw cycles should be avoided.

(Fibrin/Fibrinogen Degradation Split Products)

۱ months at -20°C

Centrifuge 2000-2500 rpm at 15 min.

(Fecal Immunochemical Test)

۱ days at 2-8°C,

۳ days at -20°C

-۲۰°C

۲ days at 2-8°C,

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۱ days at 2-8°C,

۱ months at -20°C

Be separated quickly

Slides should be prepared at the same time and dried in air and fixed.

۲ days at 2-8°C,

۳ months at -20°C

Out of light,

WB must be treated same time and store at -20°C

WB:

Frozen, Clotting, Incorrct anticoagulant

Refrigerated specimens exposed to light for more than 24 cumulative hours.

۲ days at 2-8°C,

۲ months at -20°C

Cold chain compliance during transport.

Repeated freeze-thaw cycles should be avoided.

Hemolysis

۱ weeks at 2-8°C,

۱ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Hemolysis, Icteric, Lipemic.

۱ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۱ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۷ days at 2-8°C,

۳ months at -20°C

Most sensitive test for detection of hyperandrogenemia in women and children. Acceptable test for androgen deficiency in men.

۳ months at -20°C

(Fluorescent treponemal antibody absorption test)

۷ days at 2-8°C,

۶ months at -20°C

Chem.

۷ days at 2-8°C,

۳ months at -20°C

۲ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Avoid using of OCP.

۱ months at -20°C

Repeated freeze-thaw cycles should be avoided.

The patient is not exposed to radioactive material few days before test.

(Glucose challenge test)

۶ months at -20°C

U(R&24h)

۶ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۲ months at -20°C

Avoid using of Clonidine.

(Glucose Tolerance Test)

۶ months at -20°C

۷ days at 2-8°C,

۶ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۷ days at 2-8°C,

۶ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۷ days at 2-8°C,

۶ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۱ months at -20°C

۷ days at 2-8°C,

۳ months at -20°C

Notice use of androgen & sulfasalazine drugs.

۳ months at -20°C

۳ months at -20°C

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

S

۱ weeks at

۲-۸°C,

۶ weeks < -20°C

(prefered),

or -20°C

S

PCR

۱ weeks at

۲-۸°C,

۶ weeks < -20°C

(prefered),

or -20°C

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

• Individuals at risk for HCV

Positive results require confirmation by molecular testing (eg, Hepatitis C Virus by Quantitative PCR) or Hepatitis C Virus (HCV) by Quantitative PCR with Reflex to HCV Genotype by Sequencing ).

۳ months at -20°C

S

۲ days at 2-8°C,

۳ days at -20°C,

۴ months at -70°C

(prefered)

• Do not order prior to molecular confirmation of positive HCV screen.

S

۲ days at 2-8°C,

۳ days at -20°C,

۴ months at -70°C

(prefered)

S

PCR

۲ days at 2-8°C,

۳ days at -20°C,

۴ months at -70°C

(prefered)

۷ days at 2-8°C,

۳ months at -20°C,

(dark place)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

(Human Epididymis Protein 4)

۲ months at -20°C

Not a stand-alone tumor marker for ovarian cancer screening or diagnosis. May be used with CA-125 to monitor epithelial ovarian cancer post therapy if pretreatment levels were elevated. Do not use in mucinous or germ-cell ovarian cancer.

۳ months at -20°C

Hemolysis, Icteric.

۲ months at -20°C

۲ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۲ months at -20°C

BF, Ammniotic fluid, …

۶ weeks at

۲-۸°C,

(prefered)

Frozen specimens.

BF, Ammniotic fluid, …

PCR

۶ weeks at

۲-۸°C,

۶ weeks < -20°C

(prefered)

U(R)

Colorimetry

۳ months at -20°C

Cervical

Swab,

Papule & Vesicle swab, Thin prep, …

&

Genotyping

All others: 1 ml

۳ weeks at

۲-۸°C,

۳ months at -20°C

(prefered)

Vaginal cream &

Contraceptive jel,

Douching.

۷ days at 2-8°C,

۳ months at -20°C,

Repeated freeze-thaw cycles should be avoided.

۷ days at 2-8°C,

۳ months at -20°C,

Repeated freeze-thaw cycles should be avoided.

BAL, PE, Dermal & Genital (vaginal & cervix) lesion or swab, Ammniotic fluid, Thin prep, Occular dis.

Others: 1 ml

۳ days at 2-8°C,

۳ months at -20°C

All others:

۸ hrs. RT

۳ days at 2-8°C,

۳ months at -20°C

Frozen

All others:

at 2-8°C

(prefered)

Formalinized fluids, Tissue (formalin fixes),

Clot (WB), Calcium alginate tipped, wood and gel media swab.

۲-۸°C for longer time at -20°C

CSF, …

۲ weeks at 2-8°C,

(prefered)

Clotting  specimens.

(Homovanillic Acid)

۳ months at -20°C

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

(Insulin-Like Growth Factor 1)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

CSF

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

electrophoresis

۷ days at 2-8°C,

۱ months at -20°C

U(24h):

۵ days at 2-8°C,

۱ months at -20°C

No preservative

U24h:

at 2-8°C

۳ months at -20°C

۱ months at -20°C

۳ months at -20°C

Be separated

Quickly

Hemolyzed specimens.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Pateint age & Sex.

۵ days at 2-8°C

۲ months at -20°C

۱ months at -20°C

separated prior

۲۰ min.

CSF & BF Collect on ice.

Avoid using of Cefotaxim.

۳ months at -20°C

۴ days at 2-8°C,

۱ months at -20°C

Repeated freeze-thaw cycles should be avoided.

… prior few days sample collection.

۱ months at -20°C

(dark place)

Repeated freeze-thaw cycles should be avoided.

(Geimsa)

۷ days at 2-8°C,

۱ months at -20°C

BMA, BMB

(Geimsa)

۳ months at -20°C

۳ months at -20°C

CRITICAL FROZEN

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

۳ months at -20°C

۳ months at -20°C

۳ months at -20°C

Notice using of diuretics, metronidazole, flouxitine,… drugs.

(dRVVT &

APTT-LA)

۲-۸°C,

۱ months at -20°C

۱۸ months at -70°C

Repeated freeze-thaw cycles should be avoided.

Be separated quickly.

Two time centrifugation is prefered.

at 2-8°C, -20°C

۳ months at -20°C

Avoid using estrogen, Insulin,…

Few days before test.

Hemolysis, Icteric, Lipemic.

U(R&24h)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Urine:

Specimens containing preservatives other than HCl. Specimen submitted in metal containers.

(Geimsa)

( IgG )

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

( IgM )

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

U(24h)

۷ days at 2-8°C,

۱ months at -20°C

Clotting

Urine collected within 48 hours after administration of a gadolinium (Gd) containing contrast media (may occur with MRI studies). Acid preserved urine. Specimens contaminated with blood or fecal material. Specimens transported in non-trace element free transport tube (with the exception of the original device).

۱ months at -20°C,

(dark place)

Repeated freeze-thaw cycles should be avoided.

If possible, abstain from medications for 72 hours prior to collection.

۲ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۲ months at -20°C

۲ months at -20°C

۱ months at 2-8°C,

۶ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۱ months at 2-8°C,

۶ yrs. at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

۷ days at 2-8°C,

Frozen, Clotting, Hemolysis

۷ days at 2-8°C,

Frozen, Clotting, Hemolysis

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

BF, …

BF, …

Sample,

Tissue

(Fresh & block),

BF,

Gastric juice,

Urine, Stool, …

۵ mm,

Stool: 1 gr,

Others: 1 ml

۲ weeks at -20°C

(prefered)

Uretral,

Urine,

Semen, …

۱ days at 2-8°C

In transport media

In transport media

Dry swabs,

Non-urogenital sources.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

or

Aquaporin-4 Receptor Antibody

۱ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

If possible, abstain from medications for 72 hours prior to collection.

pH: 2-3

۱ months at -20°C,

(dark place)

If possible, abstain from medications for 72 hours prior to collection.

U(R&24h)

۱ gram

۲ months at -20°C

(Stool must be frozen at once)

Urine collected with preservatives.

WB(H)

Frozen, Clotting

pH: 1.5-2

۱ months at -20°C

۷ days at 2-8°C

Frozen, Clotting, Hemolysis

LBC

۲. Instruct the patient not to douche or engage in sexual intercourse within 24 hours of the procedure.

Slides.

۲. Instruct the patient not to douche or engage in sexual intercourse within 24 hours of the procedure.

۳ months at -20°C

۲ months at -20°C

(Post Coital Test)

smear

Ana.

۲ months at -20°C

۷ days at 2-8°C,

۲ weeks at -20°C

۴ days at 2-8°C,

۱ months at -20°C

۴ days at 2-8°C,

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Icteric, Lipemic.

Ana.

U(R&24h)

۳ months at -20°C

Blood collection without tourniquet.

Icteric, Lipemic.

Stool: Formed or viscous stool.

(Serine Protease 3 Antibody)

۳ months at -20°C

۳ months at -20°C

۳ months at -20°C

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Icteric, Lipemic.

۳ months at -20°C

Avoid using Estrogen , Insulin,…

Few days before test.

U(R&24h)

۱ months at -20°C

Hemolysis

۱ months at -20°C

CRITICAL FROZEN

Repeated freeze-thaw cycles should be avoided.

Be separated quickly.

Two time centrifugation is prefered.

Coumadin, Estrogen,… ۱۰ days

before test.

۱ months at -20°C

CRITICAL FROZEN

Repeated freeze-thaw cycles should be avoided.

Be separated quickly.

Two time centrifugation is prefered.

Coumadin, Estrogen,… ۱۰ days

before test.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

CSF: Specimens should be collected prior to the intrathecal administration of contrast media.

U: Refrigerate during collection.

Urine:

Specimens collected in acid (such as those previously preserved with HCl).

۷ days at 2-8°C

Frozen, Clotting, Hemolysis

۳ months at 2-8°C

Repeated freeze-thaw cycles should be avoided.

Ejaculation 2 days before test.

۳ weeks at -20°C

Be separated quickly.

Heroine,…

۳ months at -20°C

CRITICAL FROZEN

Repeated freeze-thaw cycles should be avoided.

The sample must be frozen immediately.

Normal sodium diet (100-200 mEq/day) for at least three days. Receiving no medications known to affect renin-aldosterone system.

Supine: Specimen should be obtained between 8 a.m. and 10 a.m. (after at least two hours in supine position.

Or

Upright: Specimen should be obtained before noon (after at least two hours in upright position; seated or standing.

staining

۳ days at 2-8°C

Frozen, Clotting, Hemolysis

۳ months at -20°C

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

sample

plasma

CRITICAL FROZEN

(Sex Hormone Binding Globulin)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

U(R&24h)

۳ months at -20°C

۱ days at 2-8°C

۲ hrs. at RT,

Preservative:

۲ days at RT or

۲-۸°C

(cary blair media):

۲ days at RT or

۲-۸°C

Ether,

Direct smear

Others:

۳ days at RT,

۱ months at 2-8°C.

Added formalin 10%, stable  ۳ years.

۴ days at 2-8°C,

۲ weeks at -20°C

۱ months at -20°C

An.

۷ days at 2-8°C,

۱ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۷ days at 2-8°C,

۳ months at -20°C

۷ days at 2-8°C,

۱ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۲ months at -20°C

۳ months at -20°C

Collect between 6-10 a.m.

Use in conjunction with free testosterone in the evaluation of suspected hyperandrogenemia in women and children.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Be separated quickly.

swab

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Be separated until

۲۰-۳۰ min.

(Total iron binding capacity)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Icteric, Lipemic.

۳ months at -20°C

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

۳ months at -20°C

U(R&24h)

۲ months at -20°C

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

Abstain using of alchol before 3 days.

Indefinitely

at -20°C

Repeated freeze-thaw cycles should be avoided.

۱ months at -20°C

(Tissue Transglutaminase)

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

• May aid in monitoring adherence to gluten-free diet in celiac disease confirmed patients.

Presence of the TTG (IgA) antibody is associated with glutensensitive enteropathies such as celiac disease and dermatitis herpetiformis.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

• May aid in monitoring adherence to gluten-free diet in celiac disease confirmed patients.

The tTG IgG assay may aid in the diagnosis of gluten-sensitivity enteropathy (i.e., celiac disease, dermatitis herpetiformis) in tTG IgA negative patients with confirmed IgA deficiency. A negative tTG IgG test alone does not rule out gluten-sensitive enteropathy.

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۷ days at 2-8°C,

۳ months at -20°C

Urine:

۲ days at 2-8°C,

۲ weeks at -20°C

Repeated freeze-thaw cycles should be avoided.

Specimens with pH less than 8.0. Urine collected with acid.

۱ days at 2-8°C

۲ hrs. at RT,

۱ days at 2-8°C

at 2-8°C

An.

۴ months at -20°C

۳ months at -20°C

۳ months at -20°C

(dark place)

Be separated in refrigerated centrifuge.

Sample collection is done in cold tube, syringe.

۱ months at -20°C

(dark place)

Repeated freeze-thaw cycles should be avoided.

۲ weeks at -20°C

With preservative (20 mg oxalic acid in 1 ml serum)

۱ months at -20°C

(dark place)

Be separated quickly.

The oxalic acid preservative may interfere with other tests.

Abstain using contraceptive drugs.

۱ week at 2-8°C,

۶ months at -20°C

Repeated freeze-thaw cycles should be avoided.

۳ months at -20°C

(dark place)

abstain from alcohol for 24 hours prior to collection

With pH: 2-4,

Or

۷ days at -20°C

Abstain using coffee, cacao, chocolate,… for 72 hours prior to collection.

 (VWF ) Ag

۲ weeks at -20°C

۳ months at -20°C

CSF: Generally not recommended for the diagnosis of acute disease/encephalitis. May aid in diagnosing varicella-zoster virus vasculopathy.

۳ months at -20°C

Frozen, Clotting, Hemolysis

۳ months at -20°C

An.

۳ months at -20°C

U(R&24h)

۳ months at -20°C

Urine:

High concentrations of iodine may interfere with elemental testing. Abstinence from iodine-containing medications or contrast agents for at least 1 month prior to collecting specimens for elemental testing is recommended.

Icteric, Lipemic.

Urine:

Urine collected within 48 hours after administration of a gadolinium (Gd) containing contrast media (may occur with MRI studies). Acid preserved urine. Specimen contaminated with blood or fecal material.