به شکل جدول

Num Test name

 

Specimen type Assay method Specimen Volume (mL) Storage Temperature Transport Temperature Patient Preparation Unacceptable Conditions Ordering Recommendation
 

 

 

 

 

۱

۱۷ OH-Corticosteriods

(Ketogenic or Ketosteroids)

 

U (24h, R) Colorimetric ۱۰ ۱ week at 2-8°C

۱ months at -20°C

at -20°C The provider will instruct you, if necessary, to stop medicines that may interfere with the test. These may include:

  • Birth control pills that contain estrogen
  • Certain antibiotics
  • Glucocorticoids

 

Alkali preservatives (e.g. specimens previously preserved with NaOH) This test can help determine if the body is producing too much of the hormone, cortisol. The test may be used to diagnose Cushing syndrome. This is a disorder that occurs when the body has a high level of cortisol.
 

۲

۱۷ OH-Progesterone

 

S, PH EIA ۰.۳ ۱ week at 2-8°C

۶ months at -20°C

at 2-8°C, -20°C Avoid using medication Grossly hemolyzed , Icteric & Lipemic specimens The 17-OH progesterone test is important for all newborn babies. However, the test should also be considered for anyone who develops symptoms of congenital adrenal hyperplasia (CAH) later in life.

Keep in mind that anyone who has been diagnosed with CAH should be tested periodically so the condition can be monitored. Changes in 17-OH progesterone levels may indicate a need for an adjustment in treatment.

 

۳ ۲ME

(۲-mercaptoethanol)

S, BF Tube aggl. ۰.۴ ۱ week at 2-8°C

Longer time at -20°C

 

at 2-8°C, -20°C Grossly hemolyzed , Icteric & Lipemic specimens
۴ ۵-HIAA

(۵-Hydroxyindoleacetic Acid)

 

 

U (24h, R) EIA ۱۰ ۱ week at 2-8°C,

۲ weeks at -20°C

 

(dark place)

at 2-8°C, -20°C Patients should abstain, if possible, from medications, over-the-counter drugs, and herbal remedies for at least 72 hours prior to the test. Foods rich in serotonin (avocados, bananas, eggplant, pineapple, plums, tomatoes, walnuts) and medications that may affect metabolism of serotonin must be avoided at least 72 hours before and during collection of urine for HIAA.

 

Any sample except urine Use to diagnose carcinoid tumors and monitor disease.
۵ ACE

(Angiotensin Converting Enzyme)

 

S, BF Enzymatic ۰.۳ ۵ days at 2-8°C,

۶ months at -20°C

 

Be separated quickly.

at 2-8°C, -20°C Notice using of ACE-inhibitors, Acetate, Bromides, Captopril, Chloride, Cilazapril, EDTA, Enalapril, Fluorides, Heavy metals, Lisinopril, Nitrates, Oxalates, Perindopril, Prednisone, Propranolol, Ramipril, Trandolapril, Triiodothyronine … that affected ACE level.

 

hemolyzed , Icteric & Lipemic specimens Evaluation of patients with suspected sarcoidosis

 

Note:  Measurement of ACE activity for the evaluation of sarcoidosis is not reliable when ACE inhibitors are present. Serum ACE activity is markedly reduced in patients on ACE inhibitor therapy.

۶ Acetaminophen (Tylenol) S Photometric ۱ ۱ week at 2-8°C,

۲ months at -20°C

at 2-8°C, -20°C Plasma. Specimens collected in separator tubes, sodium fluoride/potassium oxalate, or sodium citrate anticoagulants.

 

Acetaminophen concentrations greater than 150 µg/mL at four hours after ingestion and 40 µg/mL at 12 hours after ingestion are often associated with toxicity.
۷ Acetylcholine Receptor Ab

(AChR Ab)

S EIA ۰.۵ ۲ weeks at 2-8°C,

۱ year at -20°C

 

Be separated quickly. Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Plasma. Hemolyzed, lipemic, moderately icteric serum or contaminated specimens. An acetylcholine receptor (AChR) antibody test is used to help diagnose myasthenia gravis (MG) and to distinguish it from other conditions that may cause similar symptoms, such as chronic muscle fatigue and weakness.

 

 

 

 

 

 

 

 

 

۸

Acid Phosphatase

( Prostatic)

S (Added 30 μL acetic acid 1.5 mol/L to 1 ml of serum and mix) Photometric ۰.۳ ۳ hrs. at RT

۲۴ hrs. at 2-8°C

۶ months at -20°C

 

Be separated quickly. Repeated freeze-thaw cycles should be avoided.

at -20°C •Fasting is recommended.

•Abstain using of cefotaxim few days prior to collection.

•Notice using of Alglucerase, Androgens, Clofibrate, Fluorides, goserelin, Heparins, Ketoconazole, Oxalates … that affected ACE level.

•Avoid  prostate massage & rectal exam 2 days prior to collection.

 

Plasma. Non-frozen specimens. Hemolyzed specimens. Obsolete test for prostate cancer screening; preferred test is total prostate specific antigen (PSA) in conjunction with digital rectal exam.
 

 

۹

Acid Phosphatase, Total S (Added 30 μL acetic acid 1.5 mol/L to 1 ml of serum and mix) Photometric ۰.۳ ۱ months at -20°C

CRITICAL FROZEN

 

 

Be separated quickly. Repeated freeze-thaw cycles should be avoided.

at -20°C •Fastng is recommended.

•Abstain using of cefotaxim few days prior to collection.

•Notice using of Alcohol, Anabolic steroids, Androgens, Clofibrate,  Fluorides, Oxalates, Phosphates  … that affected ACE level.

•Avoid  prostate massage & rectal exam 2 days prior to collection.

 

Plasma. Non-frozen specimens. Hemolyzed specimens. Total acid phosphatase (AP) activity may be useful when evaluating for prostate cancer, Paget’s disease, hyperparathyroidism with skeletal involvement, and Gaucher’s disease since elevations in AP activity occur in these conditions.
۱۰ ACTH

(Adrenocorticotropic Hormone)

PE CLIA ۱ ۱۴ days at -20°C

CRITICAL FROZEN

 

Be separated quickly and transport on ice. Refrigerated centrifuge must be used.

 

at -20°C Fasting. Avoid using of tourniquet. Grossly hemolyzed or lipemic specimens.

Serum, tissue or urine. Heparinized plasma.

Aids in the diagnosis of adrenal insufficiency and determining the presence of anterior pituitary tumors.
۱۱ ADA

(Adenosine Deaminase)

S, PE, CSF, BF Enzymatic ۰.۳ ۵ days at 2-8°C,

۱ months at -20°C

at 2-8°C, -20°C Hemolysis Evaluate

An ADA test may be ordered when a person has an accumulation of pleural fluid and has signs or symptoms that suggest  TB (tuberculous meningitis), such as:

 

•Chronic cough, sometimes with bloody sputum

•Fever, chills

•Night sweats

•Unexplained weight loss

•Chest pain

 

۱۲ Albumin S, PE, PH, BF,

U(R&24H)

Photometric ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C In blood: This test can help determine if a patient has liver disease or kidney disease, or if the body is not absorbing enough protein.

 

In body fluids: Aids in discriminating transudate from exudate in the evaluation of ascites.

 

۱۳ Aldolase S Enzymatic ۰.۳ ۵ days at 2-8°C,

۲ months at  -۲۰°C

 

Be separated quickly

 

at 2-8°C, -20°C Hemolysis mild&gross, Icteric gross, Lipemic gross Do not use as a stand-alone test. This non-specific test has been replaced by more specific markers for muscle or liver damage. It has largely been replaced by other enzyme tests such as CK, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) as markers of muscle or liver damage.

 

۱۴ Aldosterone

 

S,PE, U(R&24h) EIA ۰.۵, ۱۰ ۳ days at 2-8°C,

۱ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

at 2-8°C, -20°C If upright specimen is collected, patient should be seated or standing for at least 2 hours.

Abstain using of anti blood pressure, diuretic, … ۲ weeks prior to collection.

 

Hemolysis, Icteric, Lipemic,

EDTA plasma.

The combined aldosterone/renin tests are preferred

( Aldosterone/Renin Activity Ratio) or Aldosterone and Renin, Direct with Ratio  for diagnosing hyperaldosteronism.

۱۵ Alkaline Phosphatase S, PH Photometric ۰.۲ ۳ days at 2-8°C,

۲ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

at 2-8°C, -20°C Fasting, Notice use of hepatotoxic drugs Grossly hemolyzed specimens, Icteric, Lipemic.

Specimens collected in EDTA or sodium fluoride/potassium oxalate.

 

۱۶ Alpha fetoprotein (AFP) S, PE, PH, BF ECLIA, RIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

at 2-8°C, -20°C Hemolysis Surveillance and monitoring of hepatocellular carcinoma; test is less specific than one which includes AFP-L3 isoform.

 

۱۷ Alpha-1-Antitrypsin (AAT) S, PE Enzymatic ۰.۳ ۷ days at 2-8°C,

۲ months at  -۲۰°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fasting is recommended.

Avoid using of cefotaxim prior to collection.

Hemolysis, Icteric, Lipemic

 

Alpha-1 antitrypsin (AAT) testing is used to help diagnose alpha-1 antitrypsin deficiency as the cause of early onset emphysema, especially when a person does not have obvious risk factors such as smoking or exposure to lung irritants such as dust and fumes.
۱۸ ALT  (SGPT) S, PE, PH, CSF Photometric ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

at 2-8°C, -20°C Notice use hepatotoxic drugs Hemolysis, Incorrect Sodium fluoride/potassium oxalate used as an anticoagulant, Lipemic, Specimens containing cellular material.

 

۱۹ Amino Acids Quantitative PE, CSF, U(R) HPLC U: 10

Others: 1

۱ days at 2-8°C,

۱ months at -20°C

 

Plasma sample be separated quickly.

 

at 2-8°C, -20°C Adults: Fasting specimen preferred.

Infants and children: Draw specimen prior to feeding or 2-3 hours after a meal.

Urine must be fresh.

Hemolysis In blood: Diagnose and monitor aminoacidopathies (eg, PKU, MSUD).

 

In CSF: Aid in the diagnosis of selected neurotransmitter disorders (eg, glycine encephalopathy, brain serine deficiency).

 

In Urine: Screen for disorders of amino acids transport (eg, cystinuria, lysinuric protein intolerance, HHH syndrome).  Also useful to evaluate renal tubular function.

Clinical information is needed for appropriate interpretation. Additional required information includes age, gender, diet (e.g., TPN therapy), drug therapy, and family history.

 

۲۰ Ammonia (NH3) PE Photometric ۰.۵ ۲۰ min at 2-8°C,

۳ months at -20°C

 

Refrigerated Centrifuge

 

at -20°C Avoid using of tourniquet Hemolysis, Icteric, Lipemic.

Serum or blood. Non-frozen specimens. Specimens containing oxalate or citrate

•Assisting in the diagnosis of hepatic coma

 

•Investigating and monitoring treatment for in born errors of metabolism. (pecifically used to assist in diagnosing infant Reye’s syndrome)

 

•Evaluating patients with advanced liver disease

 

۲۱ Amphetamine (AMP) PH, PE, U(R) TLC ۵, ۵۰ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C
۲۲ Amylase S, PE, PH,

U(R&24H)

Photometric ۰.۳ S&U:

۷ days at 2-8°C

S: 1 month at -20°C

U: 7 days at -20°C

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fasting Hemolysis, Icteric, Lipemic.
۲۳ ANA

(Antinuclear Antibody)

S, BF IFA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. Preferred ANA screening test for connective tissue disease.
۲۴ ANCA

(Anti-Neutrophil Cytoplasmic Antibody)

S, BF IFA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. • First-line screening test for ANCA-associated vasculitis.

• A reflex panel (ANCA-Associated Vasculitis Profile (ANCA/MPO/PR-3) )is available for the workup of suspected vasculitis.

• Recommended in the differential evaluation of autoimmune diseases with renal involvement (SLE and anti-glomerular basement membrane disease.

• Recommended adjunct diagnostic tool to differentiate ulcerative colitis from Crohn disease in IBD.

 

۲۵ Androstenedione S EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

at 2-8°C, -20°C Fasting.

Sample collection 1 week before or after menstruation.

Specimen should be collected between 6-10 a.m.

Hemolysis, Icteric, Lipemic. Aids in the investigation of virilizing endocrinopathies and in managing congenital adrenal hyperplasia in conjunction with other sex steroids. Not recommended for initial evaluation of polycystic ovarian syndrome.

 

۲۶ Anti Smooth Muscle Ab (ASMA) S IFA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

at 2-8°C, -20°C Plasma. Contaminated, heat-inactivated, hemolyzed, or lipemic specimens. May be helpful in evaluating for autoimmune liver disease.

First-line panel for the evaluation of autoimmune liver disease (ALD) is ASMA, Anti Mitochondrial Ab, Anti LKM1 and ASMA titer.

 

۲۷ Anti A titer S Hemagglutination ۰.۵ ۷ days at 2-8°C,

For longer time at -20°C

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. •Evaluation of individuals with possible hypogammaglobulinemia

 

•Investigation of suspected roundworm infections

 

۲۸ Anti B titer S Hemagglutination ۰.۵ ۷ days at 2-8°C,

For longer time at -20°C

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. •Evaluation of individuals with possible hypogammaglobulinemia

 

•Investigation of suspected roundworm infections

 

۲۹ Anti Beta-2 Glycoprotein 1 Ab (IgG) S, PE EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. Acceptable initial test when antiphospholipid syndrome (APS) is strongly suspected. Order with lupus anticoagulant (LA)  and anticardiolipin (aCL) IgG and IgM  assays. May also be useful in estimating risk of thrombosis and/or pregnancy-related morbidity in patients with SLE.

 

۳۰ Anti Beta-2 Glycoprotein 1 Ab (IgM) S, PE EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۳۱ Anti- Cardiolipin Ab (IgG) S EIA ۰.۳ ۵ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Plasma or other body fluids. Contaminated, heat-inactivated, hemolyzed, or lipemic specimens. Acceptable initial test for strong suspicion of antiphospholipid syndrome. Order with lupus anticoagulant (LA)  and anti-beta-2-glycoprotein 1 (anti-β۲GP1) IgG and IgM  assays. Combined IgG and IgM cardiolipin (aCL) antibody test  is preferred.
۳۲ Anti- Cardiolipin Ab (IgM) S EIA ۰.۳ ۵ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Plasma or other body fluids. Contaminated, heat-inactivated, hemolyzed, or lipemic specimens.
۳۳ Anti Centromer Ab (IgG) S EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Plasma.  Contaminated, hemolyzed, or severely lipemic specimens. Useful for diagnosis of systemic sclerosis (SSc). Negative results do not rule out SSc. Preferred test is Systemic Scleroderma Comprehensive Panel .(Scl-70 Ab, Anti-Ribonuclear Protein U1 & U3, ANA, PM/Scl-100 Ab and RNA Polymerase III Ab)
۳۴ Anti Chlamydia trachomatis Ab (IgG) S EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Contaminated, hemolyzed, or hyperlipemic sera.
۳۵ Anti Chlamydia trachomatis Ab (IgM) S EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Contaminated, hemolyzed, or hyperlipemic sera.
۳۶ Anti DNA (ds) S EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

 

at 2-8°C, -20°C Plasma. Contaminated, hemolyzed, or severely lipemic specimens. Secondary screening for systemic lupus erythematosus (SLE) based on ANA results.
۳۷ Anti- Endomysial Ab

( IgA )

S, PE IFA ۰.۳ ۵ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

at 2-8°C, -20°C Contaminated specimens. • Acceptable single screening test for IgA-competent individuals with suspected celiac disease.

• Preferred screening test for individuals with suspected celiac disease is Celiac Disease Reflexive Cascade  or Tissue Transglutaminase (tTG) Antibody, IgA . (IgA testing recommended to identify IgA competence)

• May aid in monitoring adherence to gluten-free diet in celiac disease confirmed patients.

 

۳۸ Anti- Endomysial Ab

 ( IgG )

S, PE IFA ۰.۳ ۵ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

at 2-8°C, -20°C Contaminated specimens. •Acceptable single screening test for IgA-deficient individuals with suspected celiac disease.

• Preferred screening test is Celiac Disease Reflexive Cascade  or Tissue Transglutaminase (tTG) Antibody, IgG .  (IgA testing recommended to identify IgA deficiency (

• May aid in monitoring adherence to gluten-free diet in celiac disease confirmed patients.

 

۳۹ Anti GBM Ab

( Glomerular Basement Membrane Antibody)

S IFA ۰.۳ ۵ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C May be useful in detecting glomerular basement membrane (GBM) antibodies. Combined use of IFA and multiplex bead assay  may improve diagnostic sensitivity for disease. If positive, may be useful for monitoring treatment response.
۴۰ Anti Hydatid  Ab (IgG) (Echinococcus Antibody) S, PE EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Contaminated or severely lipemic specimens. Adjunct to other diagnostic tests (eg, imaging) for echinococcosis. Patient’s travel history is necessary to aid in test interpretation.
۴۱ Anti Insulin Ab S RIA ۰.۵ ۱ week at 2-8°C,

۲ months at -20°C

at -20°C Plasma. Hemolyzed or lipemic specimens. •Use to determine presence of antibodies to exogenous insulin analogues. Testing not recommended for patients receiving insulin >2 weeks, as insulin antibody formation may occur.

• If pursuing antibody testing to dermine autoimmune diabetes mellitus, perform at least 2 antibody tests. In most cases, use glutamic acid decarboxylase antibody in combination with another antibody test. Other antibody tests include Glutamic Acid Decarboxylase Antibody , IA-2 Antibody , Islet Cell Cytoplasmic Antibody, IgG , and Zinc Transporter 8 Antibody.

• Do not use to differentiate type 1 DM from type 2 DM, for most cases.

 

۴۲ Anti LKM1

(Liver Kidney Microsomal Type 1 Antibodies)

s IFA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Severely hemolyzed or lipemic specimens. Differential evaluation of autoimmune liver disease of unknown etiology, especially autoimmune hepatitis (AIH) of childhood onset. Use in combination with IgG testing for type1 liver cystolic antigen  when evaluating for AIH-2.
۴۳ Anti Mitochondrial Ab (AMA) S, PE IFA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C May be useful in confirming a diagnosis of primary biliary cirrhosis.
۴۴ Anti-Mullerian Hormone

(AMH)

S EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. An AMH test may be ordered in women when evaluating ovarian function, fertility issues, especially when considering assisted reproduction procedures such as in vitro fertilization (IVF), and when a health practitioner wants to determine a woman’s likelihood of entering menopause.

 

AMH may be ordered when a woman has signs and symptoms of polycystic ovarian syndrome (PCOS)

 

۴۵ Anti Platelet Ab S IFA ۰.۳ ۵ days at 2-8°C,

۳ months at -20°C

 

 

 

 

 

at 2-8°C, -20°C For newborns less than 30 days old, collect specimen from the mother. Microbially contaminated, heat-inactivated, hemolyzed, or lipemic specimens. Not recommended for the diagnosis of immune thrombocytopenic purpura (ITP). Use to detect platelet-specific antibodies in suspected fetal or neonatal alloimmune thrombocytopenia, posttransfusion purpura, or multiplatelet transfusion refractoriness.
۴۶ Anti RNP Ab

(  Ribonucleoprotein Autoantibodies)

S EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens. Anti-RNP antibodies are directed against the U1 particle of an extractable nuclear antigen (ENA) named ribonucleoprotein, which is made up of a complex of proteins and a small nuclear RNA named U1.

 

•Useful in the differential diagnosis of connective tissue diseases with or without myopathy.

 

•RNP antibody is seen in 95-100 percent of mixed connective tissue disease and is considered specific for this syndrome if other antibodies are negative; RNP is also present in 20-30 percent of systemic lupus erythematosus and 15-25 percent of progressive systemic sclerosis. RNP antigens also contain epitopes that are immunologically identical to free Smith antigens, therefore, the Smith antibody response must be considered when interpreting RNP results

 

۴۷ Anti SCL-70 Ab                     

(Anti-Scleroderma Antibodies / Topoisomerase 1)

S, PE EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Contaminated, hemolyzed, or severely lipemic specimens. Useful for diagnosis of systemic scleroderma/sclerosis (SSc). Negative results do not rule out SSc. Preferred test is Systemic Scleroderma Comprehensive Panel. (Scl-70 Ab, Anti-Ribonuclear Protein U1 & U3, ANA, PM/Scl-100 Ab and RNA Polymerase III Ab)
۴۸ Anti- Sperm Ab S IFA ۰.۳ ۵ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Room temperature specimens. Seminal fluid. As the preferred specimen type (semen) for males suspected of sperm antibodies, this test is indicated in the investigation of male infertility:

•As a follow-up test when sperm agglutination is noted in the ejaculate

•In men with a history of testicular trauma, biopsy, vasectomy reversal, genital tract infection, or

obstructive lesions of the male ductal system

 

۴۹ Anti SSA 60 (RO) Ab  

(Anti-Sjögren’s-syndrome-related antigen A)

S EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens. Order as secondary screen based on results of ANA test or if ANA IFA is negative and Sjögren syndrome, systemic lupus erythematosus (SLE), systemic sclerosis, or myositis is strongly suspected.
۵۰ Anti SSB (La) Ab S EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

 

at 2-8°C, -20°C Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens. Recommended for the differential diagnosis of SLE and Sjogren syndrome. Preferred panel contains SSA 52, SSA 60, and SSB.
۵۱ Anti- Thyroglobulin Ab S CLIA ۰.۳ ۷ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C EDTA plasma. Grossly hemolyzed or lipemic specimens. Not recommended in the initial evaluation of autoimmune thyroid disease. Most often used to evaluate potentially unreliable thyroglobulin measurements in thyroid carcinomas.

 

۵۲ Antithrombin III activity PC Photometric ۰.۵ ۸ hrs. at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C Serum. EDTA plasma , clotted or hemolyzed specimens.

 

Recommended test to detect antithrombin deficiency.
۵۳ Apolipoprotein A-1 S Imm.turb. ۰.۴ ۷ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C Freshly drawn fasting specimen. Hemolyzed specimens. Not recommended for cardiovascular disease risk assessment. Use to detect the very rare familial alpha-lipoprotein deficiency.

 

۵۴ Apolipoprotein B S Imm.turb. ۰.۴ ۷ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C Fasting specimen. Hemolyzed specimens. Acceptable non-traditional secondary cardiovascular disease risk screen for specific populations.
۵۵ APTT PC Coagulation ۱ ۲ hrs. at RT

۴ hrs. at 2-8°C

۱۴ days at -20°C

 

at RT, 2-8°C,    -۲۰°C Notice use of argatroban, heparin, heroin, … . Serum. EDTA plasma, clotted or hemolyzed specimens. Initial test for suspected bleeding disorder.
۵۶ ASCA (IgG)     

(Anti-Saccharomyces cerevisiae antibodies)

S EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

Centrifuge 1000 rpm 10 min.

 

at 2-8°C, -20°C Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens. May be useful for the diagnosis of Crohn disease. Test by itself is not diagnostic and should be used in conjunction with other parameters to confirm disease. Inflammatory Bowel Disease Differentiation Panel  is the preferred test. (ASCA IgG&IgA, ACNA  IgG)
۵۷ Ascites fluid culture Ascites fluid Aerobic & Anaerobic ۱۰ The same time must be tested…

 

at 2-8°C Avoid using antibiotic
۵۸ ASO titer  

( Streptolysin O Antibody)

S Imm.turb. ۰.۴ ۷ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C Hemolyzed specimens. Confirm a prior infection with group A Streptococcus in patients suspected of having a nonsuppurative complication such as acute glomerulonephritis (AGN) or acute rheumatic fever (ARF). DNase-B  and streptolysin O (ASO) antibody tests are generally ordered concurrently.

 

۵۹ Aspirin (Salicylate) S Photometric ۱ ۷ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C Aid in assessment of the etiology of anion gap acidosis.
۶۰ AST  (SGOT) S, PE, PH, CSF Photometric ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

at 2-8°C, -20°C Notice to using of hepatotoxic drugs Hemolysis, Incorrect Sodium fluoride/potassium oxalate used as an anticoagulant, Lipemic, Specimens containing cellular material.

 

۶۱ Barbiturates (BAR) WB, BF, U(R&24h) TLC ۵, ۵۰ ۷ days at 2-8°C,

۲ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolyzed specimens. In blood: Optimize drug therapy and monitor patient adherence. Useful for ruling out barbiturate exposure. For follow-up testing of a presumptive result, Barbiturates, Urine Quantitative is preferred.

In urine: Preferred test to follow-up presumptive results. For general screening, Barbiturates Urine Screen with Reflex to Quantitation is preferred.

 

۶۲ Bence Jones Protein U(R&24h) Chemical ۱۰ ۷ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C Aids in the diagnosis of multiple myeloma and related disorders.
۶۳ Beta-2 Microglobulin S, PE, PH, CSF, U(R&24h) CLIA ۰.۵ ۳ days at 2-8°C,

۳ months at -20°C

(U:2 months & CSF 2 weeks at -20°C)

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C S, PE: Hemolysis, Icteric, Lipemic.

 

U: Frozen

Use for risk stratification and staging of multiple myeloma, Waldenstrom macroglobulinemia, and other lymphoproliferative disorders. Aids in differentiating glomerular and tubular renal disease.
۶۴ BhCG      S, PE EIA, ECLIA,

RIA (titer)

۰.۵ ۷ days at 2-8°C,

۱ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Abstain using of chlorpromazine few days prior test (False positive) Hemolysis, Icteric, Lipemic.
۶۵ Bilirubin S, PE Photometric ۰.۵ ۵ days at 2-8°C,

۳ months at -20°C

(dark place)

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C

(dark place)

 

Notice to using of hepatotoxic drugs Hemolysis
۶۶ Bleeding Time (BT) Duke Avoid using of anticoagulant drugs
۶۷ Blood Culture WB Aerobic ۱۰% of culture medium The same time transported at 37°C at RT < 1hr Avoid using of antibiotic More than four blood culture sets per 24 hours.

 

۶۸ Blood Group & Rh WE (E) & S Hemagglutination

(cell type & back type)

۱, ۰.۵ ۱ day at RT,

۱ week at 2-8°C

 

Do not freeze red cells.

at 2-8°C Improperly or  incompletely labeled samples,  gross hemolysis, frozen, clotting

 

۶۹ Bone marrow aspiration culture WB Aerobic & Anaerobic ۱ The same time must be tested…

 

at 2-8°C Avoid using of antibiotic
۷۰ Bone marrow biopsy culture BMB Aerobic & Anaerobic The same time must be tested…

 

Avoid using of antibiotic
۷۱ Borrelia Burgdorferi Ab (IgG) S, CSF CLIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Do not order in the absence of a positive/equivocal CLIA screening test. During first 4 weeks of clinical symptoms, order concurrent IgM Western Blot. (Lyme Disease)
۷۲ Borrelia Burgdorferi Ab (IgM) S, CSF CLIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Do not order in the absence of a positive/equivocal CLIA screening test. Useful only when ordered during first 4 weeks of clinical symptoms and concurrently with IgG Western Blot. (Lyme Disease)
۷۳ Borrelia parasite WB(E) Giemsa stain ۱ ۱ days at RT,

۷ days at 2-8°C

 

at 2-8°C
۷۴ Bronchia dis. culture Bronchia dis. Aerobic ۵ The same time must be tested…

 

Avoid using of antibiotic
۷۵ Brucella Ab (IgG) S, PE, PC, CSF EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۷۶ Brucella Ab (IgM) S, PE, PC, CSF EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۷۷ Brucella culture WB Aerobic ۵ at 37°C at 37°C Avoid using of antibiotic Swabs.

Clotted blood specimens.

 

۷۸ Brucella species PCR WB(E), CSF, BM, Lymph node aspiration, BF, …

 

Qualitative ۱ ۱ days at RT,

۳ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C Incorrect anticoagulant (heparin), Clot sample, Hemolysis
۷۹ BUN S, BF Photometric ۰.۳ ۵ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

 

at 2-8°C Nephrotoxic drugs will change blood BUN level. Specimens containing sodium fluoride.
۸۰ Buprenorphine (BUP) WB, BF, U(R&24h) TLC ۵, ۵۰ ۷ days at 2-8°C,

۲ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolyzed specimens.
۸۱ C1 inhibitor

(C1-Esterase Inhibitor)

S Imm.turb. ۰.۳ ۲ days at 2-8°C,

۲ weeks at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

 

at 2-8°C, -20°C Fasting is recommended. Hemolysis, Icteric, Lipemic. Aids in diagnosis of hereditary angioedema and monitoring response to therapy.
۸۲ C3

(Complement Component 3)

S Imm.turb. ۰.۳ ۷ days at 2-8°C,

۱ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Specimens left to clot at refrigerated temperature.
۸۳ C4

(Complement Component 4)

S, PE, PH Imm.turb. ۰.۳ ۷ days at 2-8°C,

۱ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Specimens left to clot at refrigerated temperature.
۸۴ CA 125                S, PE, PH, BF ECLIA, RIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. Not a stand-alone test for ovarian cancer screening or diagnosis. May be used in the workup for and monitoring of epithelial ovarian cancer post therapy. Not recommended for breast cancer or germ-cell tumors.
۸۵ CA 15-3 S, PE, PH, BF ECLIA, RIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. Monitor therapy and identify disease recurrence in individuals with metastatic breast cancer. Do not use for diagnosis or screening of breast cancer.
۸۶ CA 19-9 S, PE, PH, BF(Biliary/Hepatic, CSF, Pancreatic, Peritoneal/Ascites, Pleural). ECLIA, RIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. In CSF: Supportive evidence in the evaluation of suspected leptomeningeal carcinomatosis

 

Body fluid tumor markers should NOT be used as sole evidence of malignancy for diagnostic purposes and should be reviewed in correlation with cytology, serum results, and other clinical evidence. Results should be interpreted with caution when considering literature-based sources of interpretive guidance, as alternative assays and/or instruments are frequently used between studies.

 

۸۷ CA 242 S, PE, BF ECLIA, RIA ۰.۵ ۳ days at 2-8°C,

For longer time at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. Aids in diagnosis of Pancreatic and Colorectal cancer with CA 19-9.
۸۸ CA 72-4 (TAG-72) S ECLIA, RIA ۰.۵ ۳ days at 2-8°C,

For longer time at -20°C

 

at 2-8°C, -20°C Breast Carcinoma
۸۹ Calcitonin

 

S ECLIA ۰.۵ ۵ days at 2-8°C,

For longer time at -20°C

 

at 2-8°C, -20°C Fasting is recommended. Tissue or urine. EDTA plasma. Grossly hemolyzed or lipemic specimens.

 

Diagnose and monitor medullary thyroid carcinoma (MTC). Secondary test to assist in diagnosing multiple endocrine neoplasia type II and familial MTC.
۹۰ Calcium S, BF, U, (R&24H) Photometric ۰.۵ ۸ days at 2-8°C,

For longer time at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fasting is recommended. Avoid using of tourniquet.

 

Do not use powdered gloves during collection.

Specimens from patients receiving EDTA therapy or Hypaque. EDTA, citrate, or oxalate.

 

 

۹۱ Calprotectin, Stool Stool Immuno chroma. ۱ gram ۱ days at 2-8°C,

 

at 2-8°C Specimens in media or preservatives. Aid in differentiation of inflammatory bowel disease (IBD) from irritable bowel syndrome (IBS) and other functional disorders of the gastrointestinal (GI) system. Aid in monitoring IBD and prediction of relapse.

(Fecal Calprotectin is an indicator of the presence of neutrophils in stool and is not specific for IBD. Other intestinal ailments including GI infections and colorectal cancer can result in elevated concentrations of calprotectin. The diagnosis of IBD cannot be established solely on the basis of a positive calprotectin result. Patients with IBD fluctuate between active and inactive stages of disease. Calprotectin results may also fluctuate. GI bleeding of as much as 100 mL per day will increase the fecal calprotectin concentration by only 15 µg/g.)

 

۹۲ Carbamazepine (Tegretol) S HPLC ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C
۹۳ CBC

(Complete Blood count)

WB(E) Fluorescece

Flow Cytometry

۱ ۴ hrs  at RT,

۱ days at 2-8°C,

at RT, at 2-8°C Frozen specimens. Clotted or grossly hemolyzed specimens.

Incorrect anticoagulant

 

۹۴ CCP Ab (IgG)

 

(Cyclic Citrullinated Peptide Antibody)

S, PE, PH EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Contaminated, hemolyzed, grossly icteric, heat-inactivated, or severely lipemic specimens. Highly specific serologic test that aids in the workup of suspected rheumatoid arthritis or undifferentiated inflammatory arthritides.  Rheumatoid Arthritis Panel  is preferred test. (CCP Ab , Rheumatoid Factor)
۹۵ CEA                      (Carcinoembryonic Antigen) S, PE, PH, BF (CSF, Pancreatic, Pericardial, Peritoneal/Ascites or Pleural fluid)

 

   ECLIA, RIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۹۶ Ceruloplasmin S, PE, PH Chemical ۰.۵ ۷ days at 2-8°C,

For longer time at -20°C

 

at 2-8°C, -20°C Fasting is recommended. Hemolysis, Icteric, Lipemic. May be used as initial screening test in Wilson disease or copper transport disorders; preferred initial testing is panel with serum ceruloplasmin, serum copper, and free (direct) serum copper  (eg, Wilson Disease Screening Panel, Serum { Copper, Copper free or direct, Ceruloplasmin}).

 

۹۷ CH50

(Total complement activity)

S Hemolysis ۱ ۲ hrs at RT,

۱۴ days at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at -20°C Specimens left to clot at 2-8°C.

 

Hemolysis

Initial screening for suspected deficiency in the classical complement pathway.
۹۸ Chloride S, PH, U(R&24h),

BF(CSF, Drain, Pancreatic, Pericardial, Peritoneal/Ascites or Pleural fluid)

 

Photometric ۱ ۲۴ hrs. at RT,

۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fasting is recommended. Specimens containing sodium fluoride/potassium oxalate as anticoagulants. Hemolyzed specimens.
۹۹ Cholesterol S, PE, PH Photometric ۰.۳ ۲۴ hrs. at RT,

۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fasting is recommended.
۱۰۰ Cholinesterase S, WB(E) Photometric ۰.۳ Serum:

۷ days at 2-8°C,

۳ months at -20°C

WB:

۳ days at 2-8°C

Serum:

at 2-8°C, -20°C

WB:

at 2-8°C

Serum:

Hemolysis, Lipemic

WB:

Hemolysis, Clotting, Frozen, Incorrect anticoagulant

 

۱۰۱ Citrate U(R&24h)

 

pH= 2

 

Enzyme

Colorimetry

۱۰ ۱ weeks at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C
۱۰۲ CK, total S, PE, PH Photometric ۰.۳ ۷ days at 2-8°C,

۱ months at -20°C

(dark place)

 

Repeated freeze-thaw cycles should be avoided.

 

 

at 2-8°C, -20°C Avoid heavy physical activity prior test. Specimens collected in potassium oxalate or sodium fluoride. Grossly hemolyzed specimens. Nonspecific indicator of muscle inflammation or damage.

 

۱۰۳ CK MB S, PE Photometric ۰.۳ ۷ days at 2-8°C,

۱ months at -20°C

(dark place)

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Avoid heavy physical activity prior test. Cardiac troponins [troponin I or troponin T] are the recommended tests for diagnosis and management of acute coronary syndrome.
۱۰۴ Clostridium difficile toxin A&B, Stool Stool EIA ۱ gram ۲ hrs at RT

۳ days at 2-8°C

 

at 2-8°C
۱۰۵ Clotting Time (CT) WB Tube method ۱ Abstain using of anti coagulant drugs
۱۰۶ CMV Ab (IgG) S, Serum from umbilical cord blood EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Plasma or urine. Contaminated, hemolyzed, or heat-inactivated specimens. Aids in discriminating between current and past cytomegalovirus infection in immunocompetent individuals.

 

۱۰۷ CMV Ab (IgM) S, Serum from umbilical cord blood EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Plasma or urine. Contaminated, hemolyzed, or heat-inactivated specimens. Aids in discriminating between current and past cytomegalovirus infection in immunocompetent individuals.

 

۱۰۸ CMV Ag (pp65)                      WB(E) DIF ۳ ۶ hrs at RT ۴ hrs. at RT Hemolysis, Clotting, Incorrect anticoagulant

 

۱۰۹ CMV PCR, Qualititative WB(E),

BAL, Serum, Amniotic fluid, CSF, Ocular fluid, Tissue, Urine

Conventional Tissue 5 mm

 

U 10 ml

 

For quatitative assay only

WB(E) is accepted

 

WB:

۸ hrs at RT,

۳ days at 2-8°C,

۱ yr at -20°C

 

Others:

۸ hrs at RT,

۳ days at 2-8°C,

۳ months at -20°C

Tissue:

Frozen

 

Others:

۲-۸°C

Formalinized fluids, Tissue (formalin fixes),

Calcium alginate tipped,

Wood and gel media swab.

Detects cytomegalovirus but does not quantify viral load. Potentially useful for specimen types other than blood.
۱۱۰ CMV PCR, Quantitative WB(E),

BAL

Real time WB:

۸ hrs at RT,

۳ days at 2-8°C,

۱ yr at -20°C

 

Others:

۸ hrs at RT,

۳ days at 2-8°C,

۳ months at -20°C

 

۲-۸°C Heparinized specimens.

Formalinized fluids

Detect and quantify cytomegalovirus (CMV).

 

۱۱۱ Cocaine (COC) U(R) Photometric ۱ ۷ days at 2-8°C,

۱ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C
۱۱۲ Cold Agglutinins S Agglutination ۰.۵ ۷ days at 2-8°C,

۱ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C Plasma or CSF. Refrigerated whole blood. Contaminated, severely hemolyzed, or lipemic, specimens.
۱۱۳ Coombs (Direct/DAT) WB(E)

 

Hemagglutination ۰.۵ ۱ days at RT,

۵ days at 2-8°C

at 2-8°C Frozen, Hemolysis, Clotting

 

Determine presence of immunoglobins and complement degradation products.
۱۱۴ Coombs (Indirect/IAT) S Hemagglutination ۰.۵ ۵ days at 2-8°C

۱ months at -20°C

 

at 2-8°C, -20°C Hemolysis
۱۱۵ Coombs Wright S, BF, CSF Agglutination ۱ ۲ days at 2-8°C,

۷ days at -20°C

 

at 2-8°C, -20°C Hemolysis
۱۱۶ Copper (Cu) S, PH, BF, U(R&24h) Photometric ۰.۵, ۱۰ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Diet, medication, and nutritional supplements may introduce interfering substances. Patients should be encouraged to discontinue nutritional supplements, vitamins, minerals, and non-essential over-the-counter medications (upon the advice of their physician)

 

Hemolysis, Icteric, Lipemic.

 

specimens that are not separated from the red cells or clot within 6 hours.

۱۱۷ Cortisol  S, U (R&24h) ECLIA, RIA ۰.۵, ۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Avoid  using of spironolactone, quinacrine,…

Abstain stress, …

 

Sampling time between 6-8 am.

 

Hemolysis, Icteric, Lipemic.

 

•Differential diagnosis of Cushing syndrome.

• Screen and diagnose primary and secondary adrenal insufficiency.

۱۱۸ C-Peptide         

(Connecting Peptide of Insulin)

S CLIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

 

at 2-8°C, -20°C Fasting specimen preferred. Hemolysis, Icteric, Lipemic.

 

Aids in the detection of insulinoma. May aid in distinguishing type 1 from type 2 diabetes mellitus (DM) in ambiguous cases. Do not use to diagnose DM.
۱۱۹ Creatinine S, PE, PH, U(R&24h) Photometric ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Avoid using of nephrotoxic drugs. Hemolysis, Icteric, Lipemic.

Specimens obtained through catheters used to infuse hyperalimentation fluid

۱۲۰ Creatinine clearance S, U(24h) Photometric ۰.۲, ۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Avoid using of nephrotoxic drugs. Hemolysis, Icteric, Lipemic.

 

Random urine collections.

۱۲۱ CRP S, BF Imm.turb. ۰.۲ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.

 

•Preferred test to detect acute phase inflammation (eg, autoimmune diseases, connective tissue disease, rheumatoid arthritis, infection, or sepsis)

•DO NOT ORDER for cardiovascular disease (CVD) risk assessment. Test for CVD risk assessment is C-Reactive Protein, High Sensitivity.

 

۱۲۲ CRP high sensitive (HSCRP) S, BF Imm.turb. ۰.۲ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.

 

Not recommended for general cardiovascular disease risk assessment in asymptomatic adults. May aid in CVD risk stratification in specific populations.
۱۲۳ Cryoglobulin S:

Clot, maintain whole blood at 37°C untile clotting is complete. Separate serum from cells using a 37°C centrifuge, if possible.

 

Qualitative Cold Precipitation ۲ Serum 2 days at RT

 

Whole blood drawn in a pre-warmed (37°C) tube and syringe.

at RT Fasting specimen required. Plasma. Serum separator tubes (SST or clot-activating tubes). Grossly hemolyzed or lipemic specimens.

 

Frozen, storage at 2-8°C

Aids in evaluation of patients with vasculitis, macroglobulinemia, or multiple myeloma in whom symptoms occur with exposure to cold.
۱۲۴ CSF culture CSF Aerobic& Anerobic ۱ The same time must be tested. at RT Abstain using antibiotic, second tube sampling is appropriate.

 

Sensitive screening test for multiple sclerosis.
۱۲۵ CSF electrophoresis (OCBs)

 

(Oligoclonal bands)

S, CSF IEF ۱, ۱ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C
۱۲۶ CSF IgG CSF Imm.turb. ۱ weeks at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C
۱۲۷ Cyclosporine WB(E) RIA ۱ ۷ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C Pre-dose (trough) levels should be drawn. Serum or plasma. Specimens left at room temperature for longer than 24 hours. Clotted specimens.

 

Optimize drug therapy and monitor patient adherence.
۱۲۸ Cystine U(R&24h) TLC ۵ ۱ months at -20°C

 

Critical Frozen

at -20°C Avoid using of penicillinamine few days prior to collection. Use for monitoring treatment of patients previously diagnosed with cystinuria.

 

Clinical information is needed for appropriate interpretation. Additional required information includes age, gender, diet (eg, TPN therapy), drug therapy, and family history.

 

۱۲۹ Cytology BF, U(formalin added )

Formalin 10%

 

Papanicolaou staining ۱۰ ۲ days at 2-8°C

Prepared slide,

۳ months at RT

Fluid at 2-8°C,

Slide at RT

۱۳۰ D-Dimer PC, PH Imm.turb. ۱ ۸ hrs at 2-8°C,

۱ months at -20°C

 

Centrifuge at 1500 rpm 15 min.

 

at 2-8°C, -20°C Serum. EDTA plasma, clotted or hemolyzed specimens.
۱۳۱ DHEAS          (Dehydroepiandrosterone Sulfate) S, PE, PH ECLIA, RIA ۰.۴ ۷ days at 2-8°C,

۲ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.

 

Indicator of adrenal androgen production. Aids in the investigation of virilizing endocrinopathies in conjunction with other sex steroids. Not recommended for initial evaluation of polycystic ovarian syndrome.
۱۳۲ Digoxin S Photometric ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Abstain using of neomysin, spironolactone,… ۷ days prior to collection.

 

Collect specimen 8-12 hours (no earlier than 6 hours) after administration of oral dose.

 

Hemolysis, Icteric, Lipemic.

 

۱۳۳ Dihydrotestosterone (DHT) S EIA ۰.۵ ۳ hrs at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C Hemolyzed or lipemic specimens.
۱۳۴ Diphtheria Ab (IgG) S EIA ۰.۳ ۷ days at 2-8°C,

For longer time at

-۲۰°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Evaluate the ability of a patient to produce antibody to pure protein vaccines after vaccination to rule out antibody deficiency.
۱۳۵ Du factor WB(E) Hemagglutination ۱ ۱ days at RT,

۷ days at at 2-8°C

 

at 2-8°C
۱۳۶ Ear dis. culture Ear dis. Aerobic The same time must be tested.

 

at RT Avoid using of antibiotic
۱۳۷ EBV Ab ( IgG ) S, PE, PH, PC, BF, CSF CLIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.

 

۱۳۸ EBV Ab ( IgM ) S, PE, PH, PC, BF, CSF CLIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.

 

۱۳۹ Ecstasy (MDMA) WB, BF, U(R&24h) TLC ۵, ۵۰ ۷ days at 2-8°C,

۲ months at -20°C

 

at 2-8°C, -20°C
۱۴۰ Entamoeba histolytica Ab (IgG)

(amebiasis)

S EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens. Aid in the detection of amebic liver abscess. Test is not useful for intestinal infection.

 

۱۴۱ Epinephrine U(R&24h) HPLC ۵ ۷ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C Abstain from emotional and mental stress, medications for 3 days pior to collection. Room temperature specimens. Specimens preserved with boric acid or acetic acid. Specimens with pH greater than 7.

 

۱۴۲ Erythropoietin (EPO) S EIA ۰.۵ ۷ days at 2-8°C,

۲ months at -20°C

 

at 2-8°C, -20°C Bone marrow aspirate. EDTA plasma. Hemolyzed specimens.

 

Initial screening test for evaluation of polycythemia. Determine eligibility for erythropoietin therapy in anemia due to chronic renal failure.
۱۴۳ ESR WB(C) Automation ۲ ۴ hrs. at RT

۱۲ hrs. at at 2-8°C

at RT,  ۲-۸°C Frozen specimens. Hemolyzed or clotted specimens. Nonspecific test used to detect inflammation associated with infections, cancers, and autoimmune diseases.

 

۱۴۴ Estradiol S, PE ECLIA, RIA ۰.۵ ۲ days at 2-8°C,

۲ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fasting Hemolysis, Icteric, Lipemic.

 

۱۴۵ Ethanol WB, BF, U(R&24h) TLC ۵, ۵۰ ۷ days at 2-8°C,

۲ months at -20°C

 

at 2-8°C, -20°C
۱۴۶ Ethosuximide (Zarontin) S HPLC ۰.۵ ۷ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C Timing of specimen collection: Pre-dose (trough) draw – At steady state concentration.

 

۱۴۷ Eye dis. culture Eye dis. Aerobic The same time must be tested.

 

at RT Avoid using of antibiotic
۱۴۸ Factor V Leiden (APCR)        PC Coagulation ۱ ۲ hrs at RT,

۱۴ days at -20°C,

۶ months at at at

-۸۰°C

 

Repeated freeze-thaw cycles should be avoided.

Be separated quickly/Two times centrifugation is preferred.

 

at -20°C Serum, clotted or hemolyzed specimens. Recommended test to detect activated protein C resistance and confirm presence of a factor V Leiden variant.
۱۴۹ Factor V Leiden (F5) R506Q mutation WB(E) PCR ۵ ۳ days at RT,

۱ week at 2-8°C

at RT, 2-8°C Hemolysis, Frozen, Clotting, Incorrect anticoagulant,

Plasma or serum; collection of specimen in sodium heparin tubes

 

Order to detect factor V Leiden variant.

 

۱۵۰ Fasciola Hepatica Ab (IgG) S EIA ۰.۳ ۲ days at 2-8°C,

۱ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.

 

۱۵۱ FDP

(Fibrin/Fibrinogen Degradation Split Products)

PC Latex agglu. ۱ ۳ days at 2-8°C,

۱ months at -20°C

 

Centrifuge 2000-2500 rpm at 15 min.

 

at 2-8°C, -20°C Serum. EDTA plasma, clotted or hemolyzed specimens For evaluating coagulopathies (eg, disseminated intravascular coagulation), D-Dimer  is the preferred test. If FDP testing is being considered, this plasma test, rather than the serum test, is the recommended test.
۱۵۲ Fecal Occult Blood

(Fecal Immunochemical Test)

Stool Immunochemical ۱ gram ۱ hrs at RT,

۱ days at 2-8°C,

۳ days at -20°C

at RT, 2-8°C,

-۲۰°C

۱۵۳ Ferritin S, PE EIA, ECLIA, RIA ۰.۳ ۴ hrs at RT,

۲ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Avoid using of iron 7 days prior to collection. Hemolysis, Icteric, Lipemic. Aids in the diagnosis of iron deficiency anemia and iron overload. Monitor treatment of hemochromatosis.
۱۵۴ Fibrinogen PC Coagulation ۰.۵ ۲ hrs at RT,

۱ days at 2-8°C,

۱ months at -20°C

 

Be separated quickly

 

at 2-8°C, -20°C Serum. EDTA plasma, clotted or hemolyzed specimens. Determine if fibrinogen deficiency is a potential cause of bleeding.

 

۱۵۵ FNA (Fine Needle Aspiration) Asp. fluid ۱ ml fluid or 3 slides Papanicolaou staining After fixation 3 months for prepared slides at RT.

 

Slides should be prepared at the same time and dried in air and fixed.

 

at RT Establish diagnosis for mass lesions accessible by fine needle aspiration.

 

۱۵۶ Folate S, WB(E) ECLIA & RIA ۰.۵, ۱ ۴ hrs at RT,

۲ days at 2-8°C,

۳ months at -20°C

Out of light,

WB must be treated same time and store at -20°C

 

at 2-8°C, -20°C Fasting S: Hemolysis, Icteric, Lipemic.

WB:

Frozen, Clotting, Incorrct anticoagulant

 

Refrigerated specimens exposed to light for more than 24 cumulative hours.

 

۱۵۷ Free BhCG S ECLIA ۰.۴ ۴ hrs at RT,

۲ days at 2-8°C,

۲ months at -20°C

 

Cold chain compliance during transport.

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Specimens left to clot at 2-8°C,

Hemolysis

۱۵۸ Free Estriol S EIA ۰.۳ ۸ hrs at RT,

۱ weeks at 2-8°C,

۱ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Plasma.

Hemolysis, Icteric, Lipemic.

Screening test for fetal aneuploidy in conjunction with other biomarkers and ultrasonography. Indicator of fetal well-being and placental function.
۱۵۹ Free T3 S, PE, PH, PC ECLIA, RIA ۰.۵ ۷ days at 2-8°C,

۱ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Notice to drug interference such as Salicylates, … Hemolysis, Icteric, Lipemic. Not recommended for routine thyroid screening.

 

۱۶۰ Free T4 S, PE, PH, PC ECLIA, RIA ۰.۵ ۷ days at 2-8°C,

۱ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Notice to drug interference such as Salicylates, … Hemolysis, Icteric, Lipemic. Not the preferred initial thyroid disorder screening test. Order following an abnormal thyroid stimulating hormone (TSH) result to diagnose thyroid disease. Order in conjunction with TSH in cases of suspected secondary hypothyroidism.
۱۶۱ Free Testosterone S EIA ۰.۵ ۸ hrs at RT,

۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Collect specimen between 6-10 a.m. Hemolysis, Icteric, Lipemic. Aids in the evaluation of suspected hypogonadism in men with a total testosterone level at the lower limit of the normal range. Not recommended for use in women and children.

 

Most sensitive test for detection of hyperandrogenemia in women and children. Acceptable test for androgen deficiency in men.

 

 

۱۶۲ FSH S, PC ECLIA, RIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۱۶۳ FTA-ABS ( IgG )  

(Fluorescent treponemal antibody absorption test)

S, PE, PH, CSF IFA ۰.۵ ۱ days at RT,

۷ days at 2-8°C,

۶ months at -20°C

 

at 2-8°C, -20°C Fasting is recommended. Contaminated, hemolyzed, or severely lipemic specimens.
۱۶۴ Fungi Direct smear & culture Skin, scalp, BF,… The same time must be tested.

 

Avoid using antibiotic & washing Culture: Blood, Catheter tips.
۱۶۵ G6PD (Quantitative& Qualititative) WB(E&H) Floures. &

Chem.

۱ days at RT,

۷ days at 2-8°C,

at 2-8°C Hemolysis, Frozen, Clotting, Incorrect anticoagulant
۱۶۶ Gabapentin S HPLC ۱ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Timing of specimen collection: Pre-dose (trough) draw – At steady state concentration.

 

Optimize drug therapy and monitor patient adherence.

 

۱۶۷ Gamma GT S Photometric ۰.۳ ۷ days at 2-8°C,

۲ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fasting is recommended.

Avoid using of OCP.

Hemolysis, Icteric, Lipemic. May be useful as an indirect marker of alcohol abuse, nonalcoholic fatty liver disease, drug intoxication, or other liver diseases.
۱۶۸ Gastrin S, PE, PH EIA & RIA ۰.۳ ۵ hrs at 2-8°C,

۱ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Patient fast for 12 hours prior to collection is recommended.

The patient is not exposed to radioactive material few days before test.

Grossly hemolyzed or lipemic specimens. Aids in diagnosis of carcinoid and gastrinoma  tumors.

 

۱۶۹ GCT     

(Glucose challenge test)

S Photometric ۰.۵ ۵ days at 2-8°C,

۶ months at -20°C

 

at 2-8°C, -20°C Fasting
۱۷۰ Glucose S, PE, BF,

U(R&24h)

Photometric ۰.۲ ۵ days at 2-8°C,

۶ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fasting Hemolysis, Icteric, Lipemic.
۱۷۱ Growth Hormone S ECLIA ۰.۲ ۲ days at 2-8°C,

۲ months at -20°C

 

at 2-8°C, -20°C Patient must be fasting and at complete rest for 30 minutes before blood collection,

 

Avoid using of Clonidine.

 

Grossly hemolyzed or lipemic specimens.
۱۷۲ GTT                 

(Glucose Tolerance Test)

S Photometric ۰.۵ ۵ days at 2-8°C,

۶ months at -20°C

 

at 2-8°C, -20°C Patient should fast for at least eight hours prior to collection of fasting specimen.

 

Use to diagnose gestational diabetes mellitus, diabetes mellitus, or impaired glucose tolerance.
۱۷۳ H. Pylori (IgA) S, PE EIA ۰.۵ ۱ days at RT,

۷ days at 2-8°C,

۶ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۱۷۴ H. Pylori (IgG) S, PE EIA ۰.۵ ۱ days at RT,

۷ days at 2-8°C,

۶ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۱۷۵ H. Pylori (IgM) S, PE EIA ۰.۵ ۱ days at RT,

۷ days at 2-8°C,

۶ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۱۷۶ H.Pylori stool Ag Stool EIA ۱ gram ۲ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C To avoid false negative results, no antibiotics, proton pump inhibitors, or a bismuth preparation should be administered to patient for 14 days prior to specimen collection.

 

Gastric specimens, swabs or tissue. Specimens in media or preservatives. As an alternative to the H. pylori urea breath test, order this test to diagnose H. pylori.
۱۷۷ Haptoglobin S, PE, PH Nephelometry ۰.۵ ۱ days at RT,

۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Fasting specimen preferred.

 

Notice use of androgen & sulfasalazine drugs.

 

Grossly hemolyzed specimens.
۱۷۸ HAV Ab (IgM) S CLIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Specimens collected in citrate-based anticoagulant. Specimens containing particulate material. Heat-inactivated, severely hemolyzed, or lipemic specimens

 

۱۷۹ HAV Ab( Total) S EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Specimens collected in citrate-based anticoagulant. Specimens containing particulate material. Heat-inactivated, severely hemolyzed, or lipemic specimens

 

۱۸۰ Hb A2 WB(E) Electrophoresis, column chromatography ۲ ۷ days at 2-8°C

 

at 2-8°C Hemolysis, Frozen, Clotting, Incorrect anticoagulant, room temperature specimens.

 

۱۸۱ Hb Electrophoresis WB(E) Capillary ۴ ۷ days at 2-8°C

 

at 2-8°C Hemolysis, Frozen, Clotting, Incorrect anticoagulant, room temperature specimens.

 

۱۸۲ Hb F WB(E) Chemical ۴ ۷ days at 2-8°C

 

at 2-8°C Hemolysis, Frozen, Clotting, Incorrect anticoagulant, room temperature specimens.

 

 

۱۸۳ HbA1c WB(E &H) Imm.turb. ۱ ۷ days at 2-8°C

 

at 2-8°C Hemolysis, Frozen, Clotting, Incorrect anticoagulant.

 

۱۸۴ HBc Ab ( IgM ) S, PE, PC EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Heparinized plasma. Specimens containing particulate material. Heat-inactivated, severely hemolyzed, or lipemic specimens.

 

Can be ordered as part of the acute hepatitis panel which includes HAV IgM, HBV core antibody IgM, HBV surface antigen, and HCV antibody to determine if patient has acute HBV infection.

 

۱۸۵ HBc Ab (Total) S, PE, PC EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

at 2-8°C, -20°C Heparinized plasma. Specimens containing particulate material. Heat-inactivated, severely hemolyzed, or lipemic specimens.

 

Determine exposure to HBV infection. May be helpful in determining which patients are at risk for HBV reactivation and would benefit from prophylactic nucleoside analog treatment prior to initiation of immunosuppression therapy.
۱۸۶ HBe Ab S, PE, PH, EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Specimens containing particulate material or collected in citrate-based anticoagulant. Heat-inactivated, grossly hemolyzed or lipemic specimens.

 

Monitor HBV therapy; order along with HBV DNA, HBV surface antigen, HBV surface antibody and HBe antigen.
۱۸۷ HBe Ag S, PE, PH, EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

Repeated freeze-thaw cycles should be avoided.

at 2-8°C, -20°C Heat-inactivated, grossly hemolyzed, or lipemic specimens. Specimens containing particulate material, or collected in citrate-based anticoagulant.

 

Monitor HBV therapy; order along with HBV DNA, HBV surface antigen, HBV surface antibody and HBe antibody.
۱۸۸ HBs Ab S, PE, PC EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Heparinized plasma. Specimens containing particulate material. Heat-inactivated , severely hemolyzed, or lipemic specimens.

 

Monitor post-liver-transplant therapy with hepatitis B immunoglobulin in HBV-positive patients and ascertain response to HBV vaccines.

 

۱۸۹ HBs Ag (Quantitative) S, PE EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Heparinized plasma. Specimens containing particulate material. Heat-inactivated, severely hemolyzed or lipemic specimens.

 

۱۹۰ HBV PCR (Qualititative) PE, Plasma (ACD),

S

PCR ۲ ۳ days at RT,

۱ weeks at

۲-۸°C,

۶ weeks < -20°C

 

at 2-8°C

(prefered),

or -20°C

Heparinized specimens.
۱۹۱ HBV  PCR (Quantitative) PE, Plasma (ACD),

S

Real time

PCR

۲ ۳ days at RT,

۱ weeks at

۲-۸°C,

۶ weeks < -20°C

 

at 2-8°C

(prefered),

or -20°C

Heparinized specimens.
۱۹۲ HCV Ab S EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

at 2-8°C, -20°C Specimens containing particulate material. Severely hemolyzed, heat-inactivated, or lipemic specimens. Heparinized plasma.

 

Preferred single screening test for:

• Individuals at risk for HCV

Positive results require confirmation by molecular testing (eg, Hepatitis C Virus by Quantitative PCR) or Hepatitis C Virus (HCV) by Quantitative PCR with Reflex to HCV Genotype by Sequencing ).

۱۹۳ HCV Ab (Riba/Western Blot) S, PE, PH Immunoblot ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۱۹۴ HCV Genotyping  PCR PE, Plasma (ACD),

S

Electrophoresisi ۲ ۴ hrs at RT,

۲ days at 2-8°C,

۳ days at -20°C,

۴ months at -70°C

at 2-8°C

(prefered)

 

Heparinized specimens. •Reflex genotyping panel to use for prognosis and treatment selection when a higher level of subtype resolution is required.

• Do not order prior to molecular confirmation of positive HCV screen.

 

۱۹۵ HCV PCR (Qualititative) PE, Plasma (ACD),

S

PCR ۲ ۴ hrs at RT,

۲ days at 2-8°C,

۳ days at -20°C,

۴ months at -70°C

 

at 2-8°C

(prefered)

 

Heparinized specimens.
۱۹۶ HCV PCR (Quantitative) PE, Plasma (ACD),

S

Real time

PCR

۲ ۴ hrs at RT,

۲ days at 2-8°C,

۳ days at -20°C,

۴ months at -70°C

 

at 2-8°C

(prefered)

 

Heparinized specimens. Preferred single confirmation test for HCV positive screen. Order only after positive HCV screen. Use to monitor chronic disease.
۱۹۷ HDL Cholesterol S, PE, PH Photometric ۰.۳ ۲۴ hrs at RT,

۷ days at 2-8°C,

۳ months at -20°C,

(dark place)

 

at 2-8°C, -20°C Fasting. Avoid using clofibrate 7 days prior sample collection. Hemolysis, Icteric, Lipemic.
۱۹۸ HDV Ab S, PE, PH, BF EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

at 2-8°C, -20°C Room temperature specimens. Specimens containing particulate material or obvious microbial contamination. Hemolyzed or lipemic specimens.

 

Diagnose HDV infection in patient with documented acute or chronic HBV and at risk for HDV infection. Consider ordering HBV IgM core antibody testing to determine whether HDV infection is a coinfection or a superinfection with HBV.
۱۹۹ HE4

(Human Epididymis Protein 4)

S, PE, PH ECLIA ۰.۵ ۳ days at 2-8°C,

۲ months at -20°C

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. HE4 is to be used as an aid in monitoring recurrence of progressive disease in patients with epithelial ovarian cancer. Serial testing for patient HE4 results should be used in conjunction with other clinical methods for monitoring ovarian cancer. Values obtained with different assay methods should not be used interchangeably.

Not a stand-alone tumor marker for ovarian cancer screening or diagnosis. May be used with CA-125 to monitor epithelial ovarian cancer post therapy if pretreatment levels were elevated. Do not use in mucinous or germ-cell ovarian cancer.

 

۲۰۰ HEV Ab S EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Specimens containing particulate material.

Hemolysis, Icteric.

 

۲۰۱ HIV Ab (I&II) S, BF EIA ۰.۵ ۵ days at 2-8°C,

۲ months at -20°C

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۲۰۲ HIV Ab (Western Blot) S, PE,PH Western Blot ۰.۵ ۵ days at 2-8°C,

۲ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۲۰۳ HIV P24 Ag S, BF EIA ۰.۳ ۵ days at 2-8°C,

۲ months at -20°C

 

at 2-8°C, -20°C
۲۰۴ HIV PCR (Qualititative) S, PE, CSF,

BF, Ammniotic fluid, …

 

PCR ۱ ۱ days at RT,

۶ weeks at

۲-۸°C,

 

at 2-8°C

(prefered)

 

Heparinized specimens.

Frozen specimens.

۲۰۵ HIV PCR (Quantitative) S, PE, CSF,

BF, Ammniotic fluid, …

Real time

PCR

۱ ۱ days at RT,

۶ weeks at

۲-۸°C,

۶ weeks < -20°C

at 2-8°C

(prefered)

 

Heparinized specimens.

 

۲۰۶ HLA B27 WB(E) Serological ۳ ۵ days at 2-8°C at 2-8°C Hemolysis, Frozen, Clotting, Heparinized specimens May assist in the diagnosis of ankylosing spondylitis. This test is not diagnostic for ankylosing spondylitis and is only suggestive of the condition if there are other clinical signs and symptoms. Test should not be performed for prenatal diagnosis of ankylosing spondylitis since a positive result is not predictive for the disorder.
۲۰۷ HLA B5 WB(E) Serological ۳ ۵ days at 2-8°C at 2-8°C Hemolysis, Frozen, Clotting, Heparinized specimens

 

Standard of care prior to abacavir therapy per FDA. Predict risk of abacavir hypersensitivity syndrome. Relevant to most populations.
۲۰۸ HLA B8 WB(E) Serological ۳ ۵ days at 2-8°C at 2-8°C Hemolysis, Frozen, Clotting, Heparinized specimens

 

۲۰۹ Homocystein  S, PE, PH,

U(R)

Enzyme

Colorimetry

۰.۵, ۱۰ ۳ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Fasting is recommended. Hemolysis, Lipemic.
۲۱۰ HPV PCR &typing Tissue,

Cervical

Swab,

Papule & Vesicle swab, Thin prep, …

 

Qualititative

&

Genotyping

Tissue: 5 mm

 

All others: 1 ml

۲ weeks at RT,

۳ weeks at

۲-۸°C,

۳ months at -20°C

 

at 2-8°C

(prefered)

 

Frozen, Formalin fixed tissue, Using anti fungal

Vaginal cream &

Contraceptive jel,

Douching.

۲۱۱ HSV Ab (IgG) S, PE, PH, BF, SCF CLIA ۲۴ hrs at RT,

۷ days at 2-8°C,

۳ months at -20°C,

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Contaminated, heat-inactivated, hemolyzed, icteric, or severely lipemic specimens.
۲۱۲ HSV Ab (IgM) S, PE, PH, BF, SCF CLIA ۲۴ hrs at RT,

۷ days at 2-8°C,

۳ months at -20°C,

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Contaminated, heat-inactivated, hemolyzed, icteric, or severely lipemic specimens. Not recommended for herpes simplex virus (HSV) testing; IgM lacks adequate predictive value for acute infection. If pursuing antibody testing, refer to HSV IgG glycoprotein types 1 and 2 antibodies . If acute HSV infection is suspected, molecular testing is preferred .
۲۱۳ HSV PCR (Qualititative) CSF, S, WB(E), Tissue,

BAL, PE, Dermal & Genital (vaginal & cervix) lesion or swab, Ammniotic fluid, Thin prep, Occular dis.

Conventional Tissue: 5 mm

Others: 1 ml

Tissue:

۳ days at 2-8°C,

۳ months at -20°C

 

All others:

۸ hrs. RT

۳ days at 2-8°C,

۳ months at -20°C

Tissue:

Frozen

 

All others:

at 2-8°C

(prefered)

 

Heparinized specimens.

Formalinized fluids, Tissue (formalin fixes),

Clot (WB), Calcium alginate tipped, wood and gel media swab.

Preferred test for detecting herpes simplex virus (HSV) infection in CSF, neonates, or when rapid diagnostic test for suspected HSV infection is necessary.
۲۱۴ HTLV Ab (I&II) S, PE, PH, BF, SCF EIA ۰.۵ Less than 5 days at

۲-۸°C for longer time at -20°C

 

at 2-8°C, -20°C
۲۱۵ HTLV PCR (Qualititative) WB(E),

CSF, …

Conventional ۱ ۲ days at RT

۲ weeks at 2-8°C,

 

at 2-8°C

(prefered)

 

Heparinized, Frozen,

Clotting  specimens.

۲۱۶ HVA

(Homovanillic Acid)

U(R & 24h) HPLC ۲۰ ۱ weeks at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Abstain from medications for 72 hours prior to collection. Specimen types other than urine. Initial test for the diagnosis and monitoring of neuroblastoma; order concurrently with urine Vanillylmandelic Acid (VMA) test.

 

۲۱۷ IgA S, PE, PH Imm.turb. ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۲۱۸ IgE S, PE, PH Imm.turb. ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۲۱۹ IGF1

(Insulin-Like Growth Factor 1)

S CLIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fating is recommended. Plasma, tissue, or urine. Grossly hemolyzed or lipemic specimens. Aids in diagnosis of growth hormone excess or deficiency disorders.

 

۲۲۰ IgG S, PE, PH,

CSF

Imm.turb ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. Aids in diagnosis of immunoglobulin disorders, including monoclonal gammopathies and immunodeficiencies.
۲۲۱ IgG1 S Nephelometry ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fating is recommended. Hemolysis, Icteric, Lipemic. Aids in diagnosis of immunodeficiencies.
۲۲۲ IgG2 S Nephelometry ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fating is recommended. Hemolysis, Icteric, Lipemic. Aids in diagnosis of immunodeficiencies.
۲۲۳ IgG3 S Nephelometry ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fating is recommended. Hemolysis, Icteric, Lipemic. Aids in diagnosis of immunodeficiencies.
۲۲۴ IgG4 S Nephelometry ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fating is recommended. Hemolysis, Icteric, Lipemic. Aids in diagnosis of immunodeficiencies.
۲۲۵ IgM S, PE, PH Imm.turb ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. Aids in diagnosis of immunoglobulin disorders, including monoclonal gammopathies and immunodeficiencies.
۲۲۶ Immunoelectrophresis S, U(24h) Capillary

electrophoresis

۱, ۱۰ S:

۷ days at 2-8°C,

۱ months at -20°C

 

U(24h):

۵ days at 2-8°C,

۱ months at -20°C

No preservative

 

at 2-8°C, -20°C

 

U24h:

at 2-8°C

Plasma Aids in the diagnosis and management of multiple myeloma and related disorders. Use to detect and quantify serum monoclonal protein.
۲۲۷ Inhibin A S EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C

 

Plasma. Hemolyzed or severely lipemic specimens.
۲۲۸ Inhibin B S EIA ۱ ۲ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C

 

Room temperature specimens. Hemolyzed or lipemic specimens. May be used for infertility evaluation. Use to differentiate ovarian tumor with normal CA-125 from stromal or mucinous epithelial tumor. May be used for monitoring recurrence of stromal ovarian tumors.

 

۲۲۹ Insulin S CLIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Be separated

Quickly

at 2-8°C, -20°C

 

Fating is recommended. Abstain using of Cimetidines, Ethanol, Chlorpropamide,… few days prior sample collection. Heparinized plasma. Vitreous or I.V. fluids.

Hemolyzed specimens.

Aids in the detection of insulinoma. Do not use to diagnose diabetes mellitus.

 

۲۳۰ Iron S, PH Photometric ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

Pateint age & Sex.

at 2-8°C, -20°C

 

Fasting, Abstain iron compound 7 days prior sample collection and OCP, Sampling should be done preferably at 8-10 am. Specimens collected from patients receiving deferoxamine (wait six hours after last dose). Specimens containing EDTA, sodium/fluoride, oxalate, or sodium citrate. Grossly hemolyzed specimens.

 

۲۳۱ Jak2 V617F Mutation PCR WB(E) Qualititative ۵ ۲۴ hrs. at RT

۵ days at 2-8°C

at 2-8°C Serum. Specimens collected in anticoagulants other than EDTA. Frozen specimens. Clotted or grossly hemolyzed specimens.

 

Aids in the workup of suspected myeloproliferative neoplasms. Detects the JAK2 V617F mutation in peripheral blood or bone marrow.
۲۳۲ Keppra (Levetiracetam) WB, BF, U(R&24h) TLC ۵, ۵۰ ۷ days at 2-8°C,

۲ months at -20°C

 

at 2-8°C, -20°C

 

Optimize drug therapy and monitor patient adherence.

 

۲۳۳ Lactate PH, BF, CSF Chemical ۱ ۱ days at 2-8°C,

۱ months at -20°C

 

separated prior

۲۰ min.

 

CSF & BF Collect on ice.

at 2-8°C, -20°C

 

Patient should be fasting and at complete rest. Patient should avoid any exercise of the arm or hand before or during collection. Draw the specimen without the use of a tourniquet or within three minutes of applying the tourniquet, but before releasing the tourniquet.

Avoid using of Cefotaxim.

 

EDTA, citrate, or iodoacetate as anticoagulants. Tubes less than half full.
۲۳۴ Lamotrigin (Lamictal) S HPLC ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C

 

Timing of specimen collection: Pre-dose (trough) draw – At steady state concentration.

 

Grossly hemolyzed specimens. Optimize drug therapy and monitor patient adherence.

 

۲۳۵ LDH S, BF, CSF Photometric ۰.۳ ۲ days at RT,

۴ days at 2-8°C,

۱ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C Avoid using of Codeine, Clofibrate,

… prior few days sample collection.

Specimens collected in EDTA, potassium oxalate, or sodium fluoride. Hemolyzed specimens.
۲۳۶ LDL Cholesterol S, PH Photometric ۰.۳ ۵ days at 2-8°C,

۱ months at -20°C

(dark place)

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C

 

Fasting Hemolysis, Icteric, Lipemic.
۲۳۷ LE Cells WB(D) Slide staining

(Geimsa)

۱۰ The same time must be tested.

 

۲۳۸ Lead WB(E) Atomic abs. ۲ ۵ days at RT,

۷ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C

 

Serum. Heparinized or clotted specimens.
۲۳۹ Leishman body Skin wound dis.

BMA, BMB

 

Slide staining

(Geimsa)

۴ slides Unlimited after fixation at RT at RT

 

Avoid using of tropical cream 3 days prior to collection.
۲۴۰ Leptospira Ab (IgG) S, CSF IFA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C

 

۲۴۱ Leptospira Ab (IgM) S, CSF IFA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C

 

۲۴۲ Levodopa (L-DOPA) S HPLC ۰.۵ ۱ months at -20°C

CRITICAL FROZEN

 

at -20°C
۲۴۳ LH S, PE ECLIA, RIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C

 

Hemolysis, Icteric, Lipemic.
۲۴۴ Lipase S, PH Photometric ۰.۵ ۵ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C

 

Fasting
۲۴۵ Lipoprotein electrophoresis S Electrophoresis ۰.۵ ۱۰ days at 2-8°C at 2-8°C Patient should be fasting for 12-15 hours. Hemolysis, Icteric, Lipemic, Heparin and frozen samples. Body fluids. Determine the Fredrickson classification of individuals with hyperlipoproteinemia. Most useful for identifying the Type III phenotype.
۲۴۶ Listeria Ab (IgG) S IFA ۰.۲ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C

 

۲۴۷ Listeria Ab (IgM) S IFA ۰.۲ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C

 

۲۴۸ Lithium S Flame Photometry ۱ ۱ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C

 

Specimens are commonly drawn approximately 12 hours after last dose of lithium taken.

Notice using of diuretics, metronidazole, flouxitine,… drugs.

Specimens collected in lithium heparin or sodium fluoride/potassium oxalate. Grossly hemolyzed specimens.

 

۲۴۹ Lupus anti coagulant PC Coagulation

(dRVVT &

APTT-LA)

۱ ۴ hrs. at RT or

۲-۸°C,

۱ months at -20°C

۱۸ months at -70°C

 

Repeated freeze-thaw cycles should be avoided.

Be separated quickly.

Two time centrifugation is prefered.

 

at RT,

at 2-8°C, -20°C

 

Serum. EDTA plasma, clotted or hemolyzed specimens.
۲۵۰ Macroprolactin S CLIA ۱ ۲ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C

 

Sample collection 3 hrs after awake. Abstain sexual activity and stress 2 days before test .

Avoid using estrogen, Insulin,…

Few days before test.

 

EDTA plasma,

Hemolysis, Icteric, Lipemic.

۲۵۱ Magnesium S, PH, BF,

U(R&24h)

Photometric ۰.۵, ۱ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C

 

Specimens collected in EDTA, oxalate/fluoride. Hemolyzed specimens.

 

Urine:

Specimens containing preservatives other than HCl. Specimen submitted in metal containers.

 

۲۵۲ Malaria parasite WB(E) Slide staining

(Geimsa)

۰.۵ Unlimited after fixation at RT

 

at RT
۲۵۳ Measles Ab (Rubeola)

( IgG )

S, BF, CSF EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C

 

plasma, urine. Contaminated, heat-inactivated, hemolyzed, lipemic or severely icteric specimens.
۲۵۴ Measles Ab (Rubeola)

( IgM )

S, BF, CSF EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C

 

Contaminated, heat-inactivated, hemolyzed, icteric, or severely lipemic specimens.
۲۵۵ Mercury (Hg) WB(E),

U(24h)

Atomic abs. ۲ ۵ days at RT,

۷ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C

 

Diet, medication, and nutritional supplements may introduce interfering substances. Patient should be encouraged to discontinue nutritional supplements, vitamins, minerals, and non-essential over-the-counter medications (upon the advice of their physician), and avoid shellfish and seafood for 48 to 72 hours. Heparin anticoagulant. Frozen specimens.

Clotting

 

Urine collected within 48 hours after administration of a gadolinium (Gd) containing contrast media (may occur with MRI studies). Acid preserved urine. Specimens contaminated with blood or fecal material. Specimens transported in non-trace element free transport tube (with the exception of the original device).

 

Preferred test for the assessment of acute mercury exposure. For chronic exposure, Mercury, Urine  is preferred.
۲۵۶ Metanephrine U(R & 24h) EIA ۵ ۷ days at 2-8°C,

۱ months at -20°C,

(dark place)

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C

 

Abstain from emotional and mental stress.

If possible, abstain from medications for 72 hours prior to collection.

Specimens preserved with boric acid or acetic acid.
۲۵۷ Methadone (MTD) WB, BF, U(R&24h) TLC ۵, ۵۰ ۷ days at 2-8°C,

۲ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C

 

۲۵۸ Methamphetamine (MET) WB, BF, U(R&24h) TLC ۵, ۵۰ ۷ days at 2-8°C,

۲ months at -20°C

 

at 2-8°C, -20°C

 

۲۵۹ Methanol WB, BF, U(R&24h) TLC ۵, ۵۰ ۷ days at 2-8°C,

۲ months at -20°C

 

at 2-8°C, -20°C For medical purposes only.  Timing of specimen collection: Dependent on time of exposure – test upon presentation to hospital.

 

Frozen
۲۶۰ Microalbumin U(R&24h) Immu.turb. ۱ ۱ weeks at RT,

۱ months at 2-8°C,

۶ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C Detect early kidney disease in those with diabetes or other risk factors (eg, hypertension).
۲۶۱ Morphine/ Opiates (MOP) U(R) Photometric ۲ ۱ weeks at RT,

۱ months at 2-8°C,

۶ yrs. at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Notice using of codeine drugs, …
۲۶۲ MPO Ab                (Myeloperoxidase Antibody) S, PE, PC, PH EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Contaminated, hemolyzed, or severely lipemic specimens. When used in conjunction with other autoantibody tests (ANCA, PR-3), may assist in evaluating suspected immune-mediated vasculitis, especially microscopic polyangiitis (MPA).

 

۲۶۳ MTHFR A1298C mutation WB(E) PCR ۵ ۳ days at RT,

۷ days at 2-8°C,

 

at 2-8°C Plasma or serum. Heparinized specimens.

Frozen, Clotting, Hemolysis

Determine genetic cause for hyperhomocysteinemia and potential sensitivity to antifolate drugs. Test is not recommended for women who have recurrent pregnancy loss, thrombophilia screening, or neural tube defect risk assessment.

 

۲۶۴ MTHFR C677T mutation WB(E) PCR ۵ ۳ days at RT,

۷ days at 2-8°C,

 

at 2-8°C Plasma or serum. Heparinized specimens.

Frozen, Clotting, Hemolysis

 

۲۶۵ Mumps Ab ( IgG ) S, CSF EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C plasma, urine. Contaminated, heat-inactivated, hemolyzed, lipemic, or severely icteric specimens. Not recommended as a stand-alone test unless testing for evidence of antibody production from vaccination.
۲۶۶ Mumps Ab ( IgM ) S, CSF EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Contaminated, hemolyzed, heat-inactivated, or severely lipemic specimens. Aid in the diagnosis of suspected mumps infection.
۲۶۷ Mycobacterium tuberculosis Culture Sput, tissue,

BF, …

Petroff ۲ days at 2-8°C at 2-8°C
۲۶۸ Mycobacterium tuberculosis Direct smear (Bacillus Koch/BK)

 

Sput, tissue,

BF, …

Ziel Neelsen ۲ days at 2-8°C at 2-8°C
۲۶۹ Mycobacterium tuberculosis PCR Respiratory

Sample,

Tissue

(Fresh & block),

BF,

Gastric juice,

Urine, Stool, …

 

Qualititative Tissue:

۵ mm,

 

Stool: 1 gr,

 

Others: 1 ml

۱ weeks at 2-8°C

۲ weeks at -20°C

at 2-8°C

(prefered)

Blood sample, Formaline added sample.
۲۷۰ Mycoplasma hominis culture Vaginl,

Uretral,

Urine,

Semen, …

PRLO broth ۶ hrs at RT,

۱ days at 2-8°C

In transport media

 

at 2-8°C

In transport media

 

Abstain using of antibiotic Specimens not in Mycoplasma/Ureaplasma transport media.

Dry swabs,

Non-urogenital sources.

 

۲۷۱ Mycoplasma Pneumoniae Ab (IgG) S, PE, PH EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Contaminated, heat-inactivated, hemolyzed, icteric, or severely lipemic specimens.

 

May aid in the diagnosis of Mycoplasma pneumoniae in patient with persistent pneumonia that is outside of the expected acute phase.

 

۲۷۲ Mycoplasma Pneumoniae Ab (IgM) S, PE, PH EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Contaminated, heat-inactivated, hemolyzed, icteric, or severely lipemic specimens.

 

May aid in the diagnosis of Mycoplasma pneumoniae in patient with persistent pneumonia that is outside of the expected acute phase.

 

۲۷۳ Nasal dis. culture Nasal dis. Aerobic The same time must be tested.

 

at 2-8°C Abstain using of antibiotic
۲۷۴ NMO Ab (IgG)                                                      (Neuromyelitis optica)

 

or

 

Aquaporin-4 Receptor Antibody

S, PE, PC, CSF IFA ۱ ۷ days at 2-8°C,

۱ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۲۷۵ Norepinephrine U HPLC ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Abstain from emotional and mental stress.

If possible, abstain from medications for 72 hours prior to collection.

 

۲۷۶ Normetanephrine U

pH: 2-3

EIA ۷ days at 2-8°C,

۱ months at -20°C,

(dark place)

 

at 2-8°C, -20°C Abstain from emotional and mental stress.

If possible, abstain from medications for 72 hours prior to collection.

 

۲۷۷ Osmolality S, Stool,

U(R&24h)

Refractometry ۱, ۵,

۱ gram

۷ days at 2-8°C,

۲ months at -20°C

(Stool must be frozen at once)

 

at 2-8°C, -20°C Formed stool.  Specimens in media or preservatives.

 

Urine collected with preservatives.

 

۲۷۸ Osmotic Fragility WB(E),

WB(H)

Hemolysis ۲, ۵ ۱ days at RT at RT Fasting Grossly hemolyzed specimens.

Frozen, Clotting

Functional testing of red blood cell sensitivity to osmotic stress; do not use to distinguish between spherocytes in hereditary spherocytosis and acquired autoimmune hemolytic anemia.

 

۲۷۹ Oxalate U(R&24h)

pH: 1.5-2

Photometric ۱۰ ۷ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C Patient should avoid ingestion of vitamin C, Orange, Lemon, Grapefruit, Tomatoes, Spinach, Strawberry , … ۳ days prior to collection.

 

Evaluate individuals with calcium oxalate renal calculi.

 

۲۸۰ PAI-1 mutation                 (Plasminogen Activator Inhibitor type 1) WB(E) PCR ۵ ۳ days at RT,

۷ days at 2-8°C

at RT Heparinized specimens.

Frozen, Clotting, Hemolysis

Screens for genetic susceptibility for venous thromboembolism (VTE) or myocardial infarction (MI) in individuals with a personal or family history of thrombotic events. Aids risk/benefit assessment for preventive or therapeutic interventions for VTE or MI.

 

۲۸۱ Pap Smear (cytology) Liquid bases. E.prep or

LBC

۷ days at RT at RT  ۱. In premenopausal patients, obtain specimens during the second half of the menstrual period to avoid contamination by obscuring blood.

۲. Instruct the patient not to douche or engage in sexual intercourse within 24 hours of the procedure.

 

Frozen Use for screening and detection of cervical cancer.

 

۲۸۲ Pap Smear (cytology) Prepared

Slides.

Betsda Unlimited after fixation at RT at RT ۱. In premenopausal patients, obtain specimens during the second half of the menstrual period to avoid contamination by obscuring blood.

۲. Instruct the patient not to douche or engage in sexual intercourse within 24 hours of the procedure.

 

Frozen Use for screening and detection of cervical cancer.

 

۲۸۳ PAPP-A S ECLIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۲۸۴ Paul-Bunnell test S Agglutination ۰.۵ ۵ days at 2-8°C,

۲ months at -20°C

 

at 2-8°C, -20°C
۲۸۵ PCT           

(Post Coital Test)

 

Vaginal dis. Direct

smear

۰.۵ Rapidly must be tested. No sexual intercourse for 3 days.
۲۸۶ Pericardial fluid culture Pericardial fluid Aerobic &

Ana.

۵ The same time must be tested.

 

at RT Abstain using of antibiotic
۲۸۷ Phencyclidine (PCP) WB, BF, U(R&24h) TLC ۵, ۵۰ ۷ days at 2-8°C,

۲ months at -20°C

 

at 2-8°C, -20°C
۲۸۸ Phenobarbital (Luminal) S HPLC ۱ ۲ days at RT,

۷ days at 2-8°C,

۲ weeks at -20°C

 

at 2-8°C, -20°C
۲۸۹ Phenytoin (Dilantin) S HPLC ۱ ۴ hrs at RT,

۴ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C Abstain using using salicylates before few days. Preferred test for therapeutic drug management in patients with renal failure or conditions that may alter albumin concentrations.
۲۹۰ Phosphorus S, PH, U Photometric ۰.۵ ۴ hrs at RT,

۴ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fasting Hemolyzed specimens. Specimens collected in sodium fluoride/potassium oxalate, citrate.

Icteric, Lipemic.

۲۹۱ Pleural fluid culture Pleural fluid Aerobic &

Ana.

۵ The same time must be tested.

 

at 2-8°C Abstain using of antibiotic
۲۹۰ Potassium S, BF, Stool,

U(R&24h)

Flame Photometry ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Patient should avoid exercise of arm or hand before and during collection.

Blood collection without tourniquet.

Specimens collected in citrate, EDTA, potassium oxalate, or sodium fluoride. Hemolyzed specimens.

Icteric, Lipemic.

 

Stool: Formed or viscous stool.

۲۹۱ PPD (Tuberculin Test) Skin test ۴۸-۷۲ hrs. after injection must be reported.
۲۹۲ PR3 Ab               

(Serine Protease 3 Antibody)

S, PE EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. When used in conjunction with other autoantibody tests (ANCA, MPO), may assist in differentiating suspected Wegener granulomatosis (WG) from other vasculitides.

 

۲۹۳ Primidone S GC ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C
۲۹۴ Pro BNP-NT ( N Terminal) S ECLIA ۱ ۶ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Hemolysis Aids in diagnosis, prognosis, and management of acute and chronic heart failure.
۲۹۵ Progesterone S, PE ECLIA, RIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Grossly hemolyzed specimens.

Icteric, Lipemic.

Aids in the workup of suspected infertility, detection of ovulation, and assessment of the luteal phase.
۲۹۶ Prolactin (PRL) S, PE ECLIA, RIA ۰.۵ ۲ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Sample collecting 3 hrs after awake. Abstain sexual activity and stress 2 days before test.

Avoid using Estrogen , Insulin,…

Few days before test.

 

Hemolysis, Icteric, Lipemic.
۲۹۷ Protein  Electrophoresis S,

U(R&24h)

Capillary ۱, ۱۰ ۷ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C Plasma.

Hemolysis

Detects and quantifies monoclonal protein (M-protein) in serum. Use in screening and monitoring of multiple myeloma and related disorders.

 

۲۹۸ Protein C PC Chromogenic ۱ ۲ hrs at RT,

۱ months at -20°C

CRITICAL FROZEN

 

Repeated freeze-thaw cycles should be avoided.

Be separated quickly.

Two time centrifugation is prefered.

 

at -20°C Abstain using Warfarine,

Coumadin, Estrogen,… ۱۰ days

before test.

Serum. EDTA plasma, clotted or hemolyzed specimens. Recommended test to detect protein C deficiency. Do not order if individual has been on warfarin in the previous 2-4 weeks.

 

۲۹۹ Protein S PC Coagulation ۱ ۲ hrs at RT,

۱ months at -20°C

CRITICAL FROZEN

 

Repeated freeze-thaw cycles should be avoided.

Be separated quickly.

Two time centrifugation is prefered.

 

at -20°C Abstain using Warfarine,

Coumadin, Estrogen,… ۱۰ days

before test.

Serum. EDTA plasma, clotted or hemolyzed specimens. Acceptable test for detecting protein S deficiency. Preferred test is Protein S Free, Antigen . Do not order if the individual has been on warfarin therapy in the previous 2-4 weeks.
۳۰۰ Protein total S, BF, U, CSF Photometric ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C S: Tourniquet application should be minimal.

 

CSF: Specimens should be collected prior to the intrathecal administration of contrast media.

 

U: Refrigerate during collection.

 

Hemolysis, Icteric, Lipemic.

 

Urine:

Specimens collected in acid (such as those previously preserved with HCl).

۳۰۱ Prothrombin G20210A mutation WB(E) PCR ۵ ۱ days at RT,

۷ days at 2-8°C

at 2-8°C Heparinized specimens.

Frozen, Clotting, Hemolysis

 

۳۰۲ PSA S, PE, PH ECLIA, RIA ۰.۵ ۲ days at RT,

۳ months at 2-8°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fating is recommended. Abstain prostate exam, heavy exercise &

Ejaculation 2 days before test.

Hemolysis, Icteric, Lipemic.

 

Preferred initial screening test for prostate cancer in conjunction with digital rectal exam. May use to monitor patients for recurrence of cancer.
۳۰۳ PT (Prothrombin Time) PC Coagulation ۱ ۲ hrs at RT,

۳ weeks at -20°C

 

Be separated quickly.

at RT, -20°C Notice use Warfaine,

Heroine,…

Serum. EDTA plasma, clotted or hemolyzed, specimens.
۳۰۴ PTH S, PE ECLIA ۰.۵ ۲ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Fasting Hemolysis, Icteric, Lipemic.

 

۳۰۵ Renin PE CLIA ۱ ۴ weeks at -20°C

CRITICAL FROZEN

 

Repeated freeze-thaw cycles should be avoided.

The sample must be frozen immediately.

at -20°C Fasting is recommended.

Normal sodium diet (100-200 mEq/day) for at least three days. Receiving no medications known to affect renin-aldosterone system.

Supine: Specimen should be obtained between 8 a.m. and 10 a.m. (after at least two hours in supine position.

Or

Upright: Specimen should be obtained before noon (after at least two hours in upright position; seated or standing.

 

Serum. Specimens collected in citrate, heparin, or oxalate. Hemolyzed specimens.
۳۰۶ Reticulocyte count WB(E) Supravital

staining

۰.۵ ۶ hrs at RT,

۳ days at 2-8°C

at RT, at 2-8°C Heparinized specimens.

Frozen, Clotting, Hemolysis

 

۳۰۷ RF (qualitative & quantitative) S, PE, PH Imm.turb. ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Hemolysis
۳۰۸ Rubella Ab( IgG) S, PE, PH, BF, CSF EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Plasma or urine. Contaminated, heat-inactivated, or hemolyzed specimens.

 

Determine seroreactivity of women of childbearing age or provide evidence of vaccination/past infection.
۳۰۹ Rubella Ab( IgM) S, PE, PH, BF, CSF EIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Plasma or urine. Contaminated, heat-inactivated, or hemolyzed specimens.
۳۱۰ Scotch Test (X1-3) Perianal

sample

Direct smear ۲ slides The same time must be tested.

 

at RT Frosted or colored tape. Specimens in media or preservatives. Stool.
۳۱۱ Semen culture Semen Aerobic The same time must be cultered.

 

Avoid using antibiotic.
۳۱۲ Semen fructose Seminal

plasma

Chemical ۰.۵ ۳ months at -20°C

CRITICAL FROZEN

at -20°C Useful when screening for obstruction or absence of the seminal vesicles in males with azoospermia.

 

۳۱۳ SHBG                          

(Sex Hormone Binding Globulin)

S, PE ECLIA or EIA ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۳۱۴ Sodium S, BF,

U(R&24h)

Flame Photometry ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Hemolysis
۳۱۵ Sputum culture Sputum Aerobic ۲ hrs at RT,

۱ days at 2-8°C

 

at 2-8°C Avoid using of antibiotic.
۳۱۶ Stone (Calculi) Analysis Urinary tract stone

 

Chemical at RT at RT
۳۱۷ Stool culture Stool Aerobic ۱ gram Unpreservative:

۲ hrs. at RT,

 

Preservative:

۲ days at RT or

۲-۸°C

 

With preservative

(cary blair media):

۲ days at RT or

۲-۸°C

 

Avoid using of antibiotic. Frozen sample
۳۱۸ Stool exam (X1-3) Stool Formalin

Ether,

Direct smear

۱ gram Bloody & mocusal sample the same time must be tested.

Others:

۳ days at RT,

۱ months at 2-8°C.

 

Added formalin 10%, stable  ۳ years.

 

at RT, at 2-8°C Frozen sample, Specimens containing barium, oil, or urine.
۳۱۹ Stool fat Stool Sudan III ۰.۵ gram ۱ hrs at RT,

۴ days at 2-8°C,

۲ weeks at -20°C

 

at RT, at 2-8°C Diapers. Specimens in media or preservatives.
۳۲۰ Sugar chromaography U(R) TLC ۱۰ ۴ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C
۳۲۱ Synovial fluid culture Synovial fluid Aerobic &

An.

۲ The same time must be cultered.

 

at RT Avoid using of antibiotic. Syringes with needle attached.
۳۲۲ T3 (total) S, PE, PH ECLIA, RIA ۰.۳ ۱ days at RT,

۷ days at 2-8°C,

۱ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Notice to hormonal treatment & gestation. Hemolysis, Icteric, Lipemic.
۳۲۳ T3 UP S, PE, PH ECLIA, RIA ۰.۳ ۱ days at RT,

۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Notice to hormonal treatment & gestation. Hemolysis, Icteric, Lipemic.
۳۲۴ T4 (total)            S, PE, PH ECLIA, RIA ۰.۳ ۱ days at RT,

۷ days at 2-8°C,

۱ months at -20°C

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Notice to hormonal treatment & gestation. Hemolysis, Icteric, Lipemic.
۳۲۵ Tacrolimus (FK506) WB(E) RIA ۲ ۷ days at 2-8°C,

۲ months at -20°C

 

at 2-8°C, -20°C Pre-dose (trough) levels should be drawn. Serum or plasma. Specimens left at room temperature for longer than 24 hours. Clotted, Hemolysed specimens.

 

Optimize drug therapy and monitor patient adherence.

 

۳۲۶ Testosterone S, PE, PH ECLIA, RIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Fasting is recommended.

 

Collect between 6-10 a.m.

Hemolysis, Icteric, Lipemic. Aids in the evaluation of suspected hypogonadism in men. Use to monitor testosterone replacement therapy.

Use in conjunction with free testosterone in the evaluation of suspected hyperandrogenemia in women and children.

 

۳۲۷ Tetanus Ab (IgG) S EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

Be separated quickly.

 

at 2-8°C, -20°C Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens. Evaluate the ability of a patient to produce antibody to pure protein vaccine after vaccination to rule out antibody deficiency.
۳۲۸ Throat culture Throat

swab

Aerobic The same time must be cultered.

 

at RT Avoid using of antibiotic.
۳۲۹ Thyroglobulin S CLIA ۰.۵ ۲ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Abstain from doing this test after FNA from thyroid, thyroid surgery and treatment with radioactive substance Hemolysis, Icteric, Lipemic.
۳۳۰ Thyroid Peroxidase               (Anti-TPO) S, PE, PH, PC ECLIA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

Be separated until

۲۰-۳۰ min.

 

at 2-8°C, -20°C Grossly hemolyzed or lipemic specimens. Not recommended for initial thyroid disorders testing. Distinguish thyroid autoimmune disorders from nonautoimmune disease or hypothyroidism.
۳۳۱ TIBC          

(Total iron binding capacity)

S Photometric ۱ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fasting Grossly hemolyzed specimens. EDTA plasma.

Icteric, Lipemic.

۳۳۲ Topamax (Topiramate) S HPLC ۱ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Timing of specimen collection: Pre-dose (trough) draw – At steady state concentration.

 

Optimize drug therapy and monitor patient adherence.

 

۳۳۳ Toxocara Ab (IgG) S, PE, PH, BF EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۳۳۴ Toxoplasma Ab (IgG) S, PE, PH, BF, CSF

 

EIA, IFA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. Detect prior exposure to T. gondii.

 

۳۳۵ Toxoplasma Ab (IgM) S, PE, PH, BF, CSF EIA, IFA ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. Detect presence of IgM antibodies to T. gondii. For male patients or nonpregnant female patients with equivocal or positive results, PCR may also be useful if a specimen can be collected from an affected body site.

 

۳۳۶ Tramadol WB, BF,

U(R&24h)

TLC ۵, ۵۰ ۷ days at 2-8°C,

۲ months at -20°C

 

at 2-8°C, -20°C
۳۳۷ Transferrin S, PE, PH Imm.turb. ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fasting specimen preferred. Hemolysis, Icteric, Lipemic.
۳۳۸ Transferrin saturation S, PE, PH Calculation ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

at 2-8°C, -20°C
۳۳۹ Triglycerides S, PH Photometric ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fasting 12 hrs.

Abstain using of alchol before 3 days.

Hemolysis, Icteric
۳۴۰ Troponin I (TnI) S, PE, PH ECLIA ۰.۵ ۷ days at 2-8°C,

Indefinitely

at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. Recommended test for the diagnosis and management of acute coronary syndrome.

 

۳۴۱ TSH S, PH ECLIA, RIA ۰.۵ ۵ days at 2-8°C,

۱ months at -20°C

 

at 2-8°C, -20°C Notice to hormonal & chemotherapy drugs. Hemolysis, Icteric, Lipemic. Preferred test for screening and monitoring of thyroid function.

 

۳۴۲ TTG (IgA)

(Tissue Transglutaminase)

S EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis, Lipemic. • Recommended single screening test for IgA-competent individuals with suspected celiac disease. (IgA testing recommended to identify IgA competence(

• May aid in monitoring adherence to gluten-free diet in celiac disease confirmed patients.

 

Presence of the TTG (IgA) antibody is associated with glutensensitive enteropathies such as celiac disease and dermatitis herpetiformis.

 

۳۴۳ TTG (IgG) S EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Hemolysis,  Lipemic. • Recommended single screening test for IgA-competent individuals with suspected celiac disease. (IgA testing recommended to identify IgA competence(

• May aid in monitoring adherence to gluten-free diet in celiac disease confirmed patients.

 

The tTG IgG assay may aid in the diagnosis of gluten-sensitivity enteropathy (i.e., celiac disease, dermatitis herpetiformis) in tTG IgA negative patients with confirmed IgA deficiency. A negative tTG IgG test alone does not rule out gluten-sensitive enteropathy.

 

۳۴۴ UIBC                               (Unsaturated Iron Binding Capacity) S Photometric ۱ ۷ days at 2-8°C,

۳ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fasting Hemolysis, Icteric, Lipemic.
۳۴۵ Urethral dis. culture Urethral dis. Aerobic The same time must be cultered.

 

Avoid using of antibiotic.
۳۴۶ Uric Acid S, PE, PH, BF, U Photometric ۰.۵ Serum:

۷ days at 2-8°C,

۳ months at -20°C

Urine:

۲ days at 2-8°C,

۲ weeks at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Abstain using Salicylates before few days. Hemolysis, Icteric, Lipemic.

 

Specimens with pH less than 8.0. Urine collected with acid.

۳۴۷ Urinalysis U(R) ۱۰ ۲ hrs. at RT,

۱ days at 2-8°C

 

at 2-8°C

 

۳۴۸ Urine culture U(R) Aerobic ۱۰ Unpreservative:

۲ hrs. at RT,

۱ days at 2-8°C

 

۲۴ hrs.

at 2-8°C

 

Avoid using of antibiotic. Urine from catheter bag. Multiple (more than one in 24 hours), 24-hour, or pooled specimens. Foley catheter tips (syringe with needle attached). Delayed transport of unpreserved urine (greater than two hours at room temperature or greater than 24 hours refrigerated).

 

Quantitative culture to identify bacterial causes of urinary tract infections.

 

۳۴۹ Vaginal dis. culture Vaginal dis. Aerobic &

An.

The same time must be cultered.

 

Avoid using of antibiotic.
۳۵۰ Valproic Acid (Depakene) S, PE, PH GC ۰.۵ ۷ days at 2-8°C,

۴ months at -20°C

 

at 2-8°C, -20°C
۳۵۱ VDRL    S, BF, CSF Flocculation ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

 

۳۵۲ Vitamin A S HPLC ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

(dark place)

 

Be separated in refrigerated centrifuge.

 

at 2-8°C, -20°C Patient should fast for 12 hours and abstain from alcohol for 24 hours prior to collection.

 

Sample collection is done in cold tube, syringe.

Hemolysis, Icteric Use for nutritional assessment of vitamin A (retinol and retinyl palmitate) in serum or plasma.

 

۳۵۳ Vitamin B12 (cobalamin) S ECLIA & RIA ۰.۵ ۲ days at 2-8°C,

۱ months at -20°C

(dark place)

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fasting Hemolysis, Icteric Aids in detection of vitamin B12 deficiency in individuals with macrocytic or unexplained anemia, or unexplained neurologic disease.

 

۳۵۴ Vitamin C S Chemical ۰.۵ ۱ hrs at RT,

۲ weeks at -20°C

With preservative (20 mg oxalic acid in 1 ml serum)

۱ months at -20°C

(dark place)

 

Be separated quickly.

The oxalic acid preservative may interfere with other tests.

 

at -20°C Fasting prefered.

Abstain using contraceptive drugs.

Hemolysis, Icteric Use for nutritional assessment of vitamin C.

 

۳۵۵

 

Vitamin D (۲۵ OH vitamin D)

 

 

S, PE, PH EIA, HPLC ۰.۵ ۱ day at RT,

۱ week at 2-8°C,

۶ months at -20°C

 

Repeated freeze-thaw cycles should be avoided.

 

at 2-8°C, -20°C Fasting Hemolysis, Icteric Preferred test to diagnose vitamin D insufficiency and monitor response to therapy. Testing is recommended only for patients at risk for vitamin D insufficiency.
۳۵۶ Vitamin E S HPLC ۷ days at 2-8°C,

۳ months at -20°C

(dark place)

 

at 2-8°C, -20°C Fasting,

abstain from alcohol for 24 hours prior to collection

Use for nutritional assessment of vitamin E (alpha- and gamma-tocopherols).

 

۳۵۷ VLDL Cholesterol S, PH Calculation

 

۳۵۸ VMA                            (Vanillylmandelic Acid) U(R&24h) Col. Chrom, ۱۰ ۷ days at 2-8°C

With pH: 2-4,

Or

۷ days at -20°C

 

at 2-8°C Abstain from medications for 72 hours prior to collection.(blood pressure drugs, guanidine analogue drugs, mono amine oxidase inhibitors)

 

Abstain using coffee, cacao, chocolate,… for 72 hours prior to collection.

 

Specimen types other than urine. Initial test for the diagnosis and monitoring of neuroblastoma. Should be ordered concurrently with urine homovanillic acid test.
۳۵۹ Von Willebrand Factor

 (VWF ) Ag

PC ELFA,EIA ۶ hrs. at RT,

۲ weeks at -20°C

at RT, -20°C Serum ,EDTA plasma, clotted or hemolyzed specimens.

 

۳۶۰ VZV Ab (IgG)                  (Varicella Zoster) S, BF, CSF EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. Provide evidence of vaccination or past infection with varicella-zoster virus.

 

CSF: Generally not recommended for the diagnosis of acute disease/encephalitis. May aid in diagnosing varicella-zoster virus vasculopathy.

 

۳۶۱ VZV Ab (IgM) S, BF, CSF EIA ۰.۳ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic. Panel that combines varicella-zoster virus IgG and IgM antibodies is preferred.
۳۶۲ Warfarine resistance gene mutation (CYP2C9, VKORC1)                 WB(E) PCR ۵ ۲ days at 2-8°C at 2-8°C Heparinized specimens.

Frozen, Clotting, Hemolysis

 

Identify individuals with inherited variants that affect metabolism and/or sensitivity to warfarin.

 

۳۶۳ Widal agglutination test S, BF Agglutination ۱ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Hemolysis, Icteric, Lipemic.
۳۶۴ Wound culture Wound dis. Aerobic &

An.

The same time must be cultered.

 

at RT Avoid using of antibiotic.
۳۶۵ Wright agglutination test (Tube) S, BF, CSF Agglutination ۰.۵ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C
۳۶۶ Zinc S, BF,

U(R&24h)

Photometric ۰.۵, ۲۰ ۷ days at 2-8°C,

۳ months at -20°C

 

at 2-8°C, -20°C Diet, medication, and nutritional supplements may introduce interfering substances. Upon the advice of their physician, patients should be encouraged to discontinue nutritional supplements, vitamins, minerals, and nonessential over-the-counter medications for one week prior to sample draw.

Urine:

High concentrations of iodine may interfere with elemental testing. Abstinence from iodine-containing medications or contrast agents for at least 1 month prior to collecting specimens for elemental testing is recommended.

 

Specimens that are not separated from the red cells or clot. Hemolyzed specimens.

Icteric, Lipemic.

 

Urine:

Urine collected within 48 hours after administration of a gadolinium (Gd) containing contrast media (may occur with MRI studies). Acid preserved urine. Specimen contaminated with blood or fecal material.

May be useful as an indicator of acute deficiency. For acute toxicity, Zinc, Urine may be a more reliable indicator of exposure.

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